A kind of tuna red meat ACE inhibitory peptide and preparation method thereof
A technology for inhibiting peptide and tuna, applied in the field of tuna red meat ACE inhibitory peptide and preparation thereof, can solve the problems of unsuitability for industrial production, high cost of blood pressure-lowering peptide, low antihypertensive activity, etc., and achieves low preparation cost and good inhibitory activity. , the effect of high efficiency
Active Publication Date: 2021-07-13
舟山领鲜海洋生物科技有限公司
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Problems solved by technology
[0003] In order to overcome the problems of high cost, low efficiency, inapplicability to industrial production, and low antihypertensive activity of existing antihypertensive peptides, the present invention proposes a tuna red meat ACE inhibitory peptide and a preparation method thereof
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Embodiment 1-15
[0041] Embodiment 1-15: a preparation method of tuna red meat ACE inhibitory peptide, comprising the following steps:
[0042] 1) Thaw the tuna red meat, dry it to remove water, then add ethyl acetate to the tuna, immerse it to degrease, rotate it, dry it, and crush it for later use;
[0043] 2) adding distilled water to the pretreated red meat powder, adjusting the pH value to 6-8, adding 1-3 wt% alkaline protease, and enzymatically hydrolyzing at 45-65°C for 4 hours to obtain an enzymolyzed solution;
[0044] 3) Heat the enzymatic hydrolysis solution in a water bath at 100°C for 10 minutes to inactivate the enzyme, then centrifuge at 4000r for 20 minutes, take the supernatant to obtain the enzymatic hydrolysis solution inactivating the enzyme, then freeze-dry to obtain the polypeptide powder, and measure the ACE inhibitory activity;
[0045]4) Use a 3.5KDa ultrafiltration membrane to carry out ultrafiltration classification on the enzymatic hydrolyzate of tuna red meat, and ...
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Abstract
A tuna red meat ACE inhibitory peptide and a preparation method thereof, relating to the field of ACE inhibitory peptide preparation, the peptide amino acid sequences of the tuna red meat ACE inhibitory peptide are Met-Trp-Asn, Met-Glu-Lys-Ser, Met-Lys-Lys- Lys‑Ser or Leu‑Pro‑Arg‑Ser, the preparation method includes the following steps: 1) thawing the tuna red meat, drying, and defatting for later use; 2) enzymolyzing the pretreated red meat powder to obtain an enzymatic hydrolyzate ;3) The enzymatic hydrolyzate was subjected to enzyme-inactivating treatment to obtain enzyme-inactivating enzymatic hydrolyzate, which was freeze-dried to obtain polypeptide powder, and the ACE inhibitory activity was measured; 4) After ultrafiltration, column chromatography, high-performance liquid chromatography purification, and amino acid sequencing, The prepared tuna red meat ACE inhibitory peptide has good inhibitory activity, low preparation cost and high efficiency, and is suitable for industrial production.
Description
technical field [0001] The invention relates to the field of preparation of ACE inhibitory peptides, in particular to a tuna red meat ACE inhibitory peptide and a preparation method thereof. Background technique [0002] The occurrence of hypertension is closely related to the disorder of renin-angiotensin system (RAS) and kallikrein-kinin system (KKS). Angiotensin-converting enzyme plays a role in regulating blood pressure by converting angiotensin Ⅰ (Ang Ⅰ) into the octapeptide angiotensin Ⅱ (Ang Ⅱ ), which has a potent vasoconstrictive effect, and by degrading bradykinin, which has a vasodilation effect. Crucial role. Antihypertensive peptides can form competitive inhibition on the substrate of angiotensin-converting enzyme, and affect the generation of Ang II, which has a strong vasoconstrictive effect, so as to achieve the purpose of lowering blood pressure. Therefore, angiotensin-converting enzyme inhibition plays an important role in antihypertensive drugs target. ...
Claims
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IPC IPC(8): C07K5/083C07K5/103A61P9/12C12P21/06C07K1/36C07K1/34C07K1/20C07K1/16
CPCA61P9/12C07K5/081C07K5/101C07K5/1013C12P21/06
Inventor 赵玉勤
Owner 舟山领鲜海洋生物科技有限公司