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Bacillus altitudinis having phosphate-solubilizing function and application thereof

A technology of bacillus highlandi and phosphate solubilizing bacteria, which is applied in the application, bacteria, organic fertilizer and other directions to achieve the effects of fast growth rate, good application prospect and strong stress resistance

Pending Publication Date: 2020-02-14
SHANDONG FOREST SCI RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is a kind of Bacillus uplander with phosphorus-dissolving function and its application. The high-efficiency phosphorus-dissolving function of Bacillus uplier can be used to solve the problem that a large amount of phosphorus in the soil cannot be directly absorbed and utilized by plants. Bacillus uplander can not only use the fixed and adsorbed phosphorus to effectively alleviate the status quo of phosphorus deficiency in the soil, but also improve the utilization rate of phosphorus fertilizer, reduce the environmental pollution caused by the use of phosphorus fertilizer, and even improve the yield and quality of crops. have important practical significance

Method used

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  • Bacillus altitudinis having phosphate-solubilizing function and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Take raw materials:

[0050] Prepare the LB liquid medium used in laboratory liquid culture, including tryptone 5 g; yeast extract 4 g; sodium chloride 8 g; distilled water 850 mL; pH adjusted to 6.9.

[0051] Configure Montina inorganic phosphorus solid medium, including glucose 10 g; (NH4)2SO4 0.6 g;

[0052] NaCl 0.2 g; KCl 0.3 g; MgSO4 7H2O 0.3g; FeSO4 7H2O 0.03g;

[0053] MnSO4·4H2O 0.03 g; Ca3(PO3)2 10 g; deionized water 1000ml, adjust the pH to 7.0.

[0054] The above raw materials were configured into LB liquid medium and Montina inorganic phosphorus solid medium.

Embodiment 2

[0056] Take raw materials:

[0057]Prepare the LB liquid medium used in laboratory liquid culture, including 10 g of tryptone; 5 g of yeast extract; 10 g of sodium chloride; 1000 mL of distilled water; and adjust the pH to 7.

[0058] Configure Montina inorganic phosphorus solid medium, including glucose 8 g; (NH4)2SO4 0.5g;

[0059] NaCl 0.5g; KCl 0.4 g; MgSO4 7H2O 0.3g; FeSO4 7H2O 0.03g;

[0060] MnSO4·4H2O 0.03 g; Ca3(PO3)2 10 g; deionized water 1000ml, adjust the pH to 7.0.

[0061] The above raw materials were configured into LB liquid medium and Montina inorganic phosphorus solid medium.

Embodiment 3

[0063] Take raw materials:

[0064] Prepare the LB liquid medium used in laboratory liquid culture, including 10 g tryptone, 5 g yeast extract, 10 g sodium chloride, 1000 mL distilled water, and adjust the pH to 7.0.

[0065] Configure Montina inorganic phosphorus solid medium, including glucose 8 g; (NH4)2SO4 0.5g;

[0066] NaCl 0.5g; KCl 0.4 g; MgSO4 7H2O 0.3g; FeSO4 7H2O 0.03g;

[0067] MnSO4·4H2O 0.03 g; Ca3(PO3)2 10 g; deionized water 1000ml, adjust the pH to 7.0.

[0068] The above raw materials were configured into LB liquid medium and Montina inorganic phosphorus solid medium.

[0069] Cultivate Bacillus highlander SM01 in the culture medium in three examples respectively, the bacterial colony shape of the bacterial strain Bacillus highlander SM01 is circular, the middle part is transparent, the color is yellowish, and the edge is not neat, wherein the raw material proportioning system in the third embodiment The obtained culture medium is the most suitable medium f...

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Abstract

The invention discloses bacillus altitudinis having a phosphate-solubilizing function and application thereof. The bacillus altitudinis is preserved in China Center for microbial culture collection management committee general microbiology on 18th, March, 2019, of which the preservation number is CGMCC No.17338. The bacillus altitudinis preferably adopts an LB liquid culture medium used in culturing of laboratory liquid and a Mongina inorganic phosphate solid culture medium used in phosphate-solubilizing bacteria. According to the bacillus altitudinis having a phosphate-solubilizing function and application thereof provided by the invention, an experiment proves that effective phosphate concentration in blank control solutions of uninoculated bacteria is much less than that in fermented liquid of bacillus altitudinis SM01, that is, the bacillus altitudinis SM01 is an efficient phosphate-solubilizing fungus. A strain of the bacillus altitudinis disclosed by the invention has the characteristics of being high in growth speed, high in phosphate-solubilizing capability, high in stress resistance, and high in capability of quickly and massively colonizing on rhizosphere of plants, and has a good application prospect.

Description

technical field [0001] The invention relates to the field of phosphorus-dissolving microbial fertilizers, in particular to a bacillus uplander with phosphorus-dissolving function and application thereof. Background technique [0002] Phosphorus is the second most important element for plant growth and development after nitrogen, accounting for about 0.2% of the dry weight of plants. It is the main component involved in energy metabolism, nucleic acid and cell synthesis, and the regulation of some enzymes. Metabolism plays an important role and is an irreplaceable component in the ecosystem. Statistics show that 40% of the cultivated land in the world is deficient in phosphorus, and 74% of the cultivated land in my country is deficient in phosphorus. The main reason is that phosphorus in the soil is mainly insoluble compounds due to immobilization Part of it cannot be directly absorbed and utilized by plants, and only 0.1% of phosphorus can be directly absorbed and utilized b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K17/14C05F11/08C12R1/07C09K101/00
CPCC05F11/08C09K17/14C09K2101/00C12N1/20C12N1/205C12R2001/07
Inventor 马丙尧马江燕马春艳高伟牛赡光马海林杜振宇刘方春李宗泰刘幸红朱升祥张淑静王霞张文馨李宜明高嘉
Owner SHANDONG FOREST SCI RES INST
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