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Method for evaluating influence of bacillus calmette guerin vaccine on neutrophil cell activity in vitro

A technology of neutrophils and BCG, applied in the biological field, can solve the problems of high risk rate and human body injury, and achieve the effects of less blood consumption, less trauma and simple operation

Active Publication Date: 2020-02-18
TONGJI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] At present, to determine the effect of BCG on cancer cells, it usually needs to be injected into the human body by perfusion method, which will have a high risk rate and cause harm to the human body

Method used

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  • Method for evaluating influence of bacillus calmette guerin vaccine on neutrophil cell activity in vitro
  • Method for evaluating influence of bacillus calmette guerin vaccine on neutrophil cell activity in vitro
  • Method for evaluating influence of bacillus calmette guerin vaccine on neutrophil cell activity in vitro

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Embodiment Construction

[0035] The implementation of the present invention is described below through specific specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific implementation modes, and various modifications or changes can be made to the details in this specification based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that, in the case of no conflict, the following embodiments and features in the embodiments can be combined with each other. It should also be understood that the terminology used in the embodiments of the present invention is for describing specific implementations, not for limiting the protection scope of the present invention. The test methods for which specific conditions are not indicated in the fo...

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Abstract

The invention discloses a method for evaluating the influence of bacillus calmette guerin vaccine on neutrophil cell activity in vitro. The method includes at least the following steps: providing a culture plate; inoculating target cells on the culture plate; preparing a bacillus calmette guerin vaccine suspension; allowing neutrophil cells and the bacillus calmette guerin vaccine suspension to act on the target cells together; and adding a detection reagent into the culture plate. The method can determine whether the neutrophil cells respond to the bacillus calmette guerin vaccine in vitro.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for evaluating the influence of BCG on the activity of neutrophils in vitro. Background technique [0002] At present, to determine the effect of BCG on cancer cells, it usually needs to be injected into the human body by perfusion, which will have a high risk rate and cause harm to the human body. The invention proposes a method for evaluating the effect of BCG on the activity of neutrophils in vitro, and whether the neutrophils respond to BCG can be determined in vitro, so as to reasonably choose whether to use BCG according to individual conditions. Contents of the invention [0003] In view of the above-mentioned shortcoming of prior art, the object of the present invention is to provide a kind of method for in vitro evaluation of BCG effect on neutrophil activity, can judge neutrophil's effect on target cells in vitro according to the activity of neutrophi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02
CPCG01N33/5047C12N2503/02G01N2500/10
Inventor 陈炳地黄鑫乐文俊崔征
Owner TONGJI UNIV