Heparin oligosaccharide and application thereof in preparation of anti-angiogenesis drugs

An anti-angiogenesis, heparin oligosaccharide technology, applied in the field of biomedicine, can solve problems such as unresearched

Inactive Publication Date: 2020-02-28
CHINA PHARM UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the anti-angiogenesis effect of heparin oligosaccharides has not been studied, which helps to elucidate the anti-tumor metastasis mechanism of heparin oligosaccharides

Method used

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  • Heparin oligosaccharide and application thereof in preparation of anti-angiogenesis drugs
  • Heparin oligosaccharide and application thereof in preparation of anti-angiogenesis drugs
  • Heparin oligosaccharide and application thereof in preparation of anti-angiogenesis drugs

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Embodiment Construction

[0020] Below in conjunction with example and accompanying drawing, the technical scheme of invention is described in detail:

[0021] A heparin oligosaccharide, the heparin is cleaved by nitrous acid cleavage method, separated and purified by Bio-gel P6 molecular sieve chromatography to obtain heparin dodecose with a molecular weight of 3200D.

[0022] An application of a heparin oligosaccharide in the preparation of an anti-angiogenesis drug; the heparin oligosaccharide can inhibit the in vitro lumen formation, proliferation and migration of endothelial cells in blood vessels.

[0023] Further, the heparin oligosaccharide acts as an FGFR2 inhibitor during anti-angiogenesis, which can down-regulate the mRNA and protein expression levels of fibroblast growth factor receptor FGFR2.

[0024] Further, the heparin oligosaccharide can inhibit the binding effect of fibroblast growth factor bFGF and its receptor FGFR2.

[0025] Further, the heparin oligosaccharides are prepared into ...

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Abstract

The invention discloses heparin oligosaccharide and application thereof in preparation of anti-angiogenesis drugs, belonging to the field of biomedicine. According to the invention, the basic fibroblast growth factor bFGF is adopted to stimulate human umbilical vein endothelial cells to establish a cell abnormality model; the influence and mechanism of heparin oligosaccharide on in-vitro angiogenesis of endothelial cells are studied through in-vitro angiogenesis, co-immunoprecipitation and other experiments, and results show that the heparin oligosaccharide destroys vascular formation of vascular endothelial cells and inhibits proliferation and migration of cells, and the heparin oligosaccharide with a concentration of 1 [mu]M has the best effect; meanwhile, the heparin oligosaccharide significantly reduces the mRNA and protein expression level of the cell surface receptor FGFR2, and inhibits the combination of bFGF and FGFR2, which indicates that FGFR2 is one of the important targetsof the anti-angiogenesis of the heparin oligosaccharide. The research can provide a new way for treating tumor growth and metastasis caused by abnormal generation of new vessels.

Description

technical field [0001] The invention relates to a heparin oligosaccharide with a molecular weight of 3200D, which has the effect of inhibiting angiogenesis of vascular endothelial cells in vitro, provides the application of the heparin oligosaccharide for preparing anti-angiogenesis drugs, and belongs to the field of biomedicine. Background technique [0002] Blood vessels provide cancer cells with nutrient and catabolite exchange and facilitate communication between primary and metastatic tumors. Therefore, angiogenesis is critical for cancer development and a potential target for cancer therapy. Studies have found that heparin and low molecular weight heparin can prolong the survival of cancer patients and have significant anti-tumor metastasis in animals; and the anti-tumor activity of heparin does not depend on its anticoagulant activity, but by inhibiting angiogenesis, blocking tumor cell adhesion etc. The proliferation and migration of vascular endothelial cells is a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10A61P35/04
CPCA61P35/04C08B37/0078
Inventor 何书英万辛雨薛金冰
Owner CHINA PHARM UNIV
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