A clean extraction process for the production of chondroitin and high-quality type II collagen from pig and bovine nasal bones

Abstract

The invention discloses a novel extraction process, in particular to a clean extraction process for producing chondroitin from pig and bovine nasal bones and co-producing high-quality type II collagen. Including the production process route of chondroitin and the production process route of high-quality type II collagen peptide; the extraction of chondroitin is carried out under long-term, room temperature and weak alkaline conditions, the purity of chondroitin is very high, and no need to use during the process Oxidative decolorization of hydrogen peroxide, very few non-chondroitin impurities, non-specific disaccharide content ≤ 4%, chondroitin yield reaches 30‑35%, meeting the needs of large-scale industrial production; the protein content of the type II collagen powder ≥90%, ash content ≤0.5%, sodium residue ≤100mg / kg, hydroxyproline ≥9%, the hygienic index is qualified, the product color is bright and white, no peculiar smell, meets the food application standard, and realizes the value of the product to the greatest extent.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a clean extraction process for producing chondroitin from pig and bovine nasal bones and co-producing high-quality type II collagen. Background technique [0002] Chondroitin sulfate is a kind of glycosaminoglycan, which belongs to acidic mucopolysaccharide, and its molecular weight is generally 20-50KDa. It is widely used in the field of dietary supplements and has a significant effect on joint maintenance and elimination of inflammation. [0003] The current industrial process for chondroitin extraction is mainly alkaline hydrolysis combined with enzymatic hydrolysis process, which can complete the extraction and purification of chondroitin in a short time. However, this process has the following disadvantages: 1) the purity of chondroitin is low, which requires repeated refining and purification, resulting in more impurities in the finished product of chondroitin, dark...

AI Technical Summary

Problems solved by technology

However, this process has the following disadvantages: 1) the purity of chondroitin is low, which requires repeated refining and purification, resulting in more impurities in the finished product of chondroitin, dark color, and degradation of some chondroitin, resulting in more non- Chondroitin impurities, such as keratan sulfate, etc., lead to low enzymolysis liquid phase content (non-specific disaccharides are greater than 10%), and the quality is difficult to guarantee; 2) High-quality collagen peptides cannot be co-produced. Collagen peptide is light brown in color, has a pungent smell, exceeds the standard for microorganisms, and has a protein content of ≤90% and ash content of ≥5%. It cannot be used in the food field and is generally sold as animal feed

3) Due to the complete enzymatic hydrolysis of all cartilage in the traditional process, a large amount of biological enzymes are required, and the production cost is high

4) In the production process, a large amount of hydrogen peroxide is used for oxidation. During the long-term oxidation process, the structure of chondroitin undergoes structural transformation, and the purity decreases, and a large amount of hydrogen peroxide remains in the product, which is not conducive to human health.

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