Clean extraction process of chondroitin and high-quality type II collagen coproduction using pig and cattle nasal bones

Abstract

The invention discloses a novel extraction process and particularly relates to a clean extraction process of chondroitin and high-quality type II collagen coproduction using pig and cattle nasal bones. The clean extraction process includes a chondroitin production process route and a high-quality type II collagen production process route. The clean extraction process has the advantages that the chondroitin is extracted through long-time extraction at room temperature and under a weak alkali condition, the extracted chondroitin is quite high in purity, oxidation decoloring using hydrogen peroxide in the extraction process is not needed, the chondroitin is extremely few in non-chondroitin impurities, the non-specificity disaccharide content of the chondroitin is not more than 4%, the yield of the chondroitin reaches 30-35%, and the requirements of large-scale industrial production are satisfied; the protein content of the type II collagen powder is not less than 90%, the ash content of the type II collagen powder is not more than 0.5%, the sodium residue of the type II collagen powder is not more than 100mg/kg, the hydroxyproline content of the type II collagen powder is not less than 9%, the type II collagen powder satisfies sanitation requirements, bright white in color, free of peculiar smell and meets food application standards, and product value is achieved to the maximum extent.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a clean extraction process for producing chondroitin from pig and bovine nasal bones and co-producing high-quality type II collagen. Background technique [0002] Chondroitin sulfate is a kind of glycosaminoglycan, which belongs to acidic mucopolysaccharide, and its molecular weight is generally 20-50KDa. It is widely used in the field of dietary supplements and has a significant effect on joint maintenance and elimination of inflammation. [0003] The current industrial process for chondroitin extraction is mainly alkaline hydrolysis combined with enzymatic hydrolysis process, which can complete the extraction and purification of chondroitin in a short time. However, this process has the following disadvantages: 1) the purity of chondroitin is low, which requires repeated refining and purification, resulting in more impurities in the finished product of chondroitin, dark...

AI Technical Summary

Problems solved by technology

However, this process has the following disadvantages: 1) the purity of chondroitin is low, which requires repeated refining and purification, resulting in more impurities in the finished product of chondroitin, dark color, and degradation of some chondroitin, resulting in more non- Chondroitin impurities, such as keratan sulfate, etc., lead to low enzymolysis liquid phase content (non-specific disaccharides are greater than 10%), and the quality is difficult to guarantee; 2) High-quality collagen peptides cannot be co-produced. Collagen peptide is light brown in color, has a pungent smell, exceeds the standard for microorganisms, and has a protein content of ≤90% and ash content of ≥5%. It cannot be used in the food field and is generally sold as animal feed

3) Due to the complete enzymatic hydrolysis of all cartilage in the traditional process, a large amount of biological enzymes are required, and the production cost is high

4) In the production process, a large amount of hydrogen peroxide is used for oxidation. During the long-term oxidation process, the structure of chondroitin undergoes structural transformation, and the purity decreases, and a large amount of hydrogen peroxide remains in the product, which is not conducive to human health.

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