Application of microRNA induced open type DNA fluorescent nano-robot in tumors and construction method of microRNA induced open type DNA fluorescent nano-robot

Abstract

The invention belongs to the technical field of gene engineering, and discloses an application of a tumor marker microRNA induced open type DNA nano-robot in tumor cells and a construction method of the tumor marker microRNA induced open type DNA nano-robot. The method comprises the following steps: firstly, combining the tumor marker microRNA induced open type DNA nano-robot with a metal nano-cluster synthesized in situ by taking DNA as a template; constructing a novel fluorescent nano-robot, and achieving the functions of label-free and modification-free imaging of tumor cells and growth inhibition of the tumor cells by utilizing the functions of fluorescence imaging and cell growth inhibition of the metal nano-cluster. According to the invention, the tumor marker microRNA induced open type DNA nano-robot is employed, and the construction mode is not reported, so the invention provides a new thought for the rational design of a functionalized DNA nano-robot, and provides a new tool for the fluorescence imaging technology; the multifunctional nano-robot is used for imaging and inhibiting tumor cells and is more novel.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a method for constructing a microRNA-induced opening type DNA fluorescent nanorobot and its application in the integration of diagnosis and treatment of tumor cells. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: [0003] In the 1850s, J.D.Watson and F.H.C.Crick published the double helix structure model of DNA in Nature, and suggested that DNA is the genetic template for inheriting life. In 1983, Seeman used DNA to build nucleic acid nanostructures for the first time, indicating that DNA not only carries the important genetic information of life, but also can be used as a component for building nanomaterials, thus a new science-DNA nanotechnology was born (Seeman, 2010 ). DNA nanorobot is one of the most rapidly developing directions. It refers to the use of DNA's accurate complementary ...

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Problems solved by technology

[0007] (1) The driving components of DNA nanorobots reported in the literature, such as DNA, cannot be recycled, which limits their efficiency;

[0008] (2) The fluorescent signals required for the realization of functions such as characterization, tracking and imaging of DNA nanorobots reported in the literature need to be labeled with fluorescent groups on the DNA backbone, but the price is expensive, which limits its wide application;

[0009] (3) The functions of DNA nanorobots reported in the literature are relatively single, which limits their wide application

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