A construction method, bacterial strain and application of Aeromonas verkirea attenuated strain

Abstract

The present invention relates to Aeromonas victorii, which was preserved in the China Center for Type Culture Collection, referred to as CCTCC, on July 24, 2019, and the preservation number is CCTCC NO: M 2019580. The virulence of the mutant strain of Vernerundii of the present application is greatly reduced, and a good attenuation effect is achieved, which provides a possibility for making a live attenuated vaccine of Aeromonas vernerii.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a method for constructing an attenuated strain of Aeromonas vernerii, a strain and an application thereof. Background technique [0002] Aeromonas victorii belongs to the Aeromonas family, the genus Aeromonas, and is a Gram-negative short bacillus. It is ubiquitous in fresh water, sewage, sea water and even soil. Aeromonas verdeii is a virulent pathogenic bacteria. The pathogenic process mainly includes adhesion-invasion-colonization in the host body and toxin secretion, during which a series of virulence factors are produced, such as aerolysin, enterotoxin, etc. ) and adhesion factors, etc. These virulence factors play a pivotal role in aquatic animals, livestock and poultry, and even human infection. [0003] In recent years, an increasing number of cases have shown that Aeromonas victorii has become an important human-fish pathogenic bacteria, which is widely distributed, ...

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Problems solved by technology

[0004] At present, we mainly rely on antimicrobial drugs to prevent and control Aeromonas victorii, but drug control methods directly lead to the formation of drug-resistant pathogens, and at the same time cause many environmental problems such as drug residues and environmental pollution.

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