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Tightly linked caps molecular markers of p3g and c3g synthetic genes in colored barley

A molecular marker, barley technology, applied in the field of genetic analysis, can solve undiscovered problems

Active Publication Date: 2020-10-30
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no report on the sequence information of closely linked CAPS molecular markers of barley P3G and C3G synthesis genes / genetic loci

Method used

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  • Tightly linked caps molecular markers of p3g and c3g synthetic genes in colored barley
  • Tightly linked caps molecular markers of p3g and c3g synthetic genes in colored barley
  • Tightly linked caps molecular markers of p3g and c3g synthetic genes in colored barley

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Obtainment of P3G and C3G Synthesis Major QTL in Barley Grain Tightly Linked CAPS Molecular Marker BACS2H

[0044] The purple barley RUSSIA68 was used as the female parent and the colorless barley Gairdner was used as the male parent to obtain hybrid F1, F1 was self-crossed to obtain F2, and 120 individuals were randomly selected from F2 to form the F2 genetic verification population.

[0045] 1. Design of InDel molecular marker BAID2H and parental polymorphism test

[0046] According to the literature (Xiao-Wei Zhang, Qian-Tao Jiang, Yu-Ming Wei, et al. Inheritance analysis and mapping of quantitative trait loci (QTL) controlling individual anthocyanin compounds in purple barley (Hordeum vulgare L.) grains. Plos one, 2017, 12:e0183704) reported the main effect QTL PBG.ant-2H for the synthesis of P3G and C3G in colored barley grains. Based on the genome information of cultivated barley Hordeum vulgare cv.Morex, gene prediction analysis was performed on the fla...

Embodiment 2

[0054] Example 2 The application test of molecular marker BACS2H in the selection of barley P3G and C3G synthesis main effect QTL PBG.ant-2H

[0055] 1. Extraction, qualitative and quantitative analysis of anthocyanins

[0056] The grains of the F2 verification population were collected and placed in a ventilated box at 28°C for two weeks until assayed. Based on the literature (Abdel-Aaland Hucl 2007), an improved method was used to extract the anthocyanins in the grains. The specific operation process was as follows: the grains were pulverized by a ball mill and sieved through a 0.2 mm sieve. The anthocyanin extract was added to the flour at a ratio of 10:1 (V / M), mixed evenly and the pH value was adjusted to 1. The mixture was shaken and mixed at 250 rpm for 24 hours at 4° C. on a shaker, and then centrifuged at 10,000 g for 25 minutes. The supernatant was transferred to a clean centrifuge tube as the sample to be tested. Anthocyanin standard substance was dissolved and s...

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Abstract

The invention relates to the technical field of genetic analysis, in particular to a CAPS molecular marker closely linked to P3G and C3G synthesis genes in colored barley, and provides detection primers for the molecular marker. The present invention provides a genetic distance of 0.0cM between the molecular marker and the PBG.ant-2H site. Therefore, barley varieties or lines with P3G and C3G synthetic main QTLs can be screened out more accurately, and applying them to the early generation selection of barley breeding can further improve the accuracy of screening work in breeding.

Description

technical field [0001] The invention relates to the technical field of genetic analysis, in particular to a CAPS molecular marker closely linked to P3G and C3G synthesis genes in colored barley. Background technique [0002] Colorful barley is rich in anthocyanins, which has important potential value to human health, and is an important material for the development of functional food and health nutrition products, and its antioxidant and anti-mutation effects can be detected not only in barley grains, but also in its It can also be detected in fermentation broth. Studies have shown that different anthocyanins have different functions and different health value, and the composition of anthocyanins in barley with different colors or even barley with similar colors may be quite different. Peonidin-3-glucoside (Peonidin-3-glucoside, P3G) and cyanidin-3-glucoside (Cyanidin-3-glucoside, C3G) are ubiquitous important anthocyanin components. Therefore, research on P3G and C3G synt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/686C12N15/11
CPCC12Q1/6895C12Q1/686C12Q2600/13C12Q2600/156C12Q2521/301
Inventor 张晓伟刘春吉梅时勇刘淳劼
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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