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Application of all-trans retinoic acid to mesenchymal stem cell polarization regulation

A technology of all-trans retinoic acid and stromal cells, which is applied in the application field of all-trans retinoic acid in the differentiation regulation of mesenchymal stem cells

Pending Publication Date: 2020-03-20
深圳市蓝思人工智能医学研究院 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although a large number of surface molecules have been used to define mesenchymal stem cells, the mesenchymal stem cells defined by these surface molecules are still a group of highly heterogeneous cell groups, not a single cell group

Method used

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  • Application of all-trans retinoic acid to mesenchymal stem cell polarization regulation
  • Application of all-trans retinoic acid to mesenchymal stem cell polarization regulation
  • Application of all-trans retinoic acid to mesenchymal stem cell polarization regulation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0031] 1. Test method

[0032] 1. All-trans retinoic acid regulates the number of bone marrow mesenchymal stem cells in mice

[0033] In this example, commercially purchased healthy C57BL / 6 mice were used for intraperitoneal injection test, and the number of mouse bone marrow mesenchymal stem cells after the test was detected by flow cytometry. details as follows:

[0034] C57BL / 6 mice were equally divided into three groups with 8 mice in each group.

[0035] The first group is the test group. The mice are intraperitoneally injected with all-trans retinoic acid, once every other day, and the injected dose is 100 μL of all-trans retinoic acid solution with a concentration of 10 μmol / L. The mice were sacrificed after 7 injections, and the number and proportion of mouse bone marrow mesenchymal stem cells were analyzed by flow cytometry. Wherein, the all-trans retinoic acid solution is prepared by dissolving all-trans retinoic acid powder in the organic solvent DMSO (dimethyl s...

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PUM

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Abstract

The application of the invention discloses an application of all-trans retinoic acid to mesenchymal stem cell polarization regulation. The application of all-trans retinoic acid to mesenchymal stem cell polarization regulation specially comprises the application of the all-trans retinoic acid to restraining of polarization of mesenchymal stem cells to osteoblasts and adipocyte. The application research confirms that the all-trans retinoic acid can restrain the polarization of mesenchymal stem cells to the osteoblast and the polarization. The application provides a new function application of the all-trans retinoic acid based on the application, the polarization regulation of the mesenchymal stem cells is researched, and polarization potential of the mesenchymal stem cells is adjusted and controlled directionally. The invention provides a new tool and an adjusting means.

Description

technical field [0001] The present application relates to the field of mesenchymal stem cells, in particular to an application of all-trans retinoic acid in regulating the differentiation of mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells are a highly heterogeneous group of stromal stem cells. At present, a set of recognized surface marker molecules are used to define mesenchymal stem cells. Human mesenchymal cells do not express hematopoietic cell-related molecules such as CD45, CD34, CD14 and co-stimulatory molecules CD80, CD86, etc., mainly express CD44, Surface molecules such as CD90, CD73, and CD71. Mouse-derived mesenchymal stem cells also do not express hematopoietic-related molecules such as CD45, Ter119, and endothelial cell-related molecules such as CD31, but mainly express surface molecules such as PDGFRα, SCA-1, and CD44. Although a large number of surface molecules have been used to define mesenchymal stem cells, the mesenchymal st...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0663C12N2500/38
Inventor 赵萌娄琪牟丽莎陆赢蒲祖辉
Owner 深圳市蓝思人工智能医学研究院
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