Adhesive hemostatic antibacterial hydrogel and preparation method thereof

An antibacterial water and adhesive technology, applied in bandages, medical science, etc., can solve the problems of full-thickness wounds that are difficult to repair, threaten people's lives, and affect health, achieve antibacterial tissue repair and regeneration, great application potential, and promote tissue regeneration. The effect of repair and regeneration

Active Publication Date: 2021-12-24
BEOGENE BIOTECH GUANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While most skin defects heal quickly and efficiently within 1 or 2 weeks, extensive full-thickness wounds are often difficult to repair, which can have serious health consequences and even threaten people's lives

Method used

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  • Adhesive hemostatic antibacterial hydrogel and preparation method thereof
  • Adhesive hemostatic antibacterial hydrogel and preparation method thereof
  • Adhesive hemostatic antibacterial hydrogel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A GelMA / 2% HA-DA / AMP-01 hydrogel, the hydrogel comprising the following components in mass percent: hyaluronic acid modified dopamine 2%, methacrylated gelatin 10%, antibacterial peptide 0.005 %, photoinitiator 0.1%, and the balance is deionized water.

[0037] The preparation method of hyaluronic acid-modified dopamine (HA-DA) in the hydrogel is as follows: Weigh 1g of hyaluronic acid and dissolve it in 100mL deionized water, and then pass through nitrogen gas. After the hyaluronic acid is completely dissolved, dissolve 500mg of EDC and 250mg of NHS were slowly added into the solution, and after stirring for 20min, 600mg of dopamine hydrochloride was added to the mixed solution, and then the pH of the reaction system was monitored and adjusted to between 5 and 6 with a concentration of 0.1mol / L sodium hydroxide or hydrochloric acid solution, Then react at room temperature for 12 hours, dialyze with distilled water for 2 to 3 days (molecular weight cut-off: 10000), and ...

Embodiment 2

[0041] A GelMA / 2% HA-DA / AMP-02 hydrogel, the hydrogel comprising the following components in mass percent: hyaluronic acid modified dopamine 2%, methacrylated gelatin 10%, antibacterial peptide 0.025 %, photoinitiator 0.1%, and the balance is deionized water.

[0042] The preparation method of hyaluronic acid modified dopamine and methacrylated gelatin is the same as that in Example 1.

[0043] The preparation method of this hydrogel is: 0.2g GelMA (10wt.%), 0.04gHA-DA (2wt.%), AMP (500μg / mL, namely 0.025wt.%) and 0.002g LAP photoinitiator (0.1 wt.%) was dissolved in 2ml of deionized water and irradiated with ultraviolet light for 2 minutes to obtain GelMA / 2%HA-DA / AMP-02 hydrogel.

Embodiment 3

[0045] A GelMA / 2% HA-DA / AMP-03 hydrogel, the hydrogel comprising the following components in mass percent: hyaluronic acid modified dopamine 2%, methacrylated gelatin 10%, antibacterial peptide 0.05 %, photoinitiator 0.1%, and the balance is deionized water.

[0046] The preparation method of hyaluronic acid modified dopamine and methacrylated gelatin is the same as that in Example 1.

[0047] The preparation method of this hydrogel is: with 0.2g GelMA (10wt.%), 0.04g HA-DA (2wt.%), AMP (1000 μ g / mL, i.e. 0.05wt.%) and 0.002g LAP photoinitiator ( 0.1wt.%) was dissolved in 2ml of deionized water and irradiated with ultraviolet light for 2min to obtain GelMA / 2%HA-DA / AMP-03 hydrogel.

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Abstract

The invention relates to an adhesive hemostatic and antibacterial hydrogel, the hydrogel comprises the following components in mass percentage: 0.5%-4% of hyaluronic acid modified dopamine, 1%-20% of methacrylated gelatin %, 0.005% to 0.05% of antibacterial peptide, 0.01% to 0.5% of photoinitiator, and the balance is deionized water. The invention utilizes the unique functions of methacrylated gelatin, hyaluronic acid-modified dopamine and antibacterial peptide, and synthesizes an adhesive hemostatic and antibacterial hydrogel based on methacrylic acid gelatin. The hydrogel of the present invention realizes the dual functions of antibacterial and promoting tissue repair and regeneration, and exhibits adjustable gel time, suitable swelling in vitro, appropriate degradation, good tissue adhesion, and adjustable fluid flow Denaturation performance and excellent compression and stretching performance; the hydrogel of the present invention has drug sustained-release capability, and has good hemocompatibility and cell compatibility.

Description

technical field [0001] The invention relates to an adhesive hemostatic and antibacterial hydrogel and a preparation method thereof, belonging to the technical field of medical biomaterials. Background technique [0002] Skin tissue is the largest, most exposed, and most fragile tissue in the human body. Once skin tissue is damaged, the repair process appears to be very complex, including four important overlapping and complex stages: hemostasis, inflammation, proliferation, and remodeling / maturation. Although most skin defects can heal quickly and effectively within 1 or 2 weeks, extensive full-thickness wounds are often difficult to repair, which has serious health effects and even threatens people's lives. Therefore, various biomaterials have been developed as wound dressings, such as nanofibers, sponges, foams, and hydrogels. Among these materials, injectable polymer hydrogels have become an attractive option due to their soft extracellular matrix (ECM)-like properties,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L26/00
CPCA61L26/008A61L26/0066A61L26/0038A61L26/0023A61L26/009A61L2300/404A61L2300/604A61L2300/412A61L2300/252
Inventor 胡圣学蓝咏刘玉冯龙宝
Owner BEOGENE BIOTECH GUANGZHOU
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