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Vitamin B1 and vitamin C concentration rapid detection method based on liquid chromatography-tandem mass spectrometry technology

A tandem mass spectrometry and liquid chromatography technology, applied in the detection field of water-soluble vitamins, can solve the problems of long detection time, unstable results, long detection time, etc., and achieves strong specificity, improved detection time and effect, and good repeatability. Effect

Inactive Publication Date: 2020-03-27
合肥谱佳医学检验实验室有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

It detects multi-dimensional tablets, but the detection method combines a variety of technologies, which is relatively complicated, and the detection time is relatively long. It can be seen from its spectrum that it takes at least 20 minutes
[0004] Some existing methods for detecting the concentration of vitamin B1 and vitamin C are generally basic ion mass spectrometers, which take a long time to detect and the results are unstable. There is an urgent need for a fast, simple, accurate and efficient detection method for the concentration of vitamin B1 and vitamin C

Method used

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  • Vitamin B1 and vitamin C concentration rapid detection method based on liquid chromatography-tandem mass spectrometry technology
  • Vitamin B1 and vitamin C concentration rapid detection method based on liquid chromatography-tandem mass spectrometry technology
  • Vitamin B1 and vitamin C concentration rapid detection method based on liquid chromatography-tandem mass spectrometry technology

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Embodiment Construction

[0037] The embodiments of the present invention are described in detail below. This embodiment is implemented on the premise of the technical solution of the present invention, and detailed implementation methods and specific operating procedures are provided, but the protection scope of the present invention is not limited to the following implementation example.

[0038] The specific detection process of this embodiment is as follows:

[0039] 1. Preparation of standard products

[0040] 1. Preparation of standard product stock solution: Accurately weigh the appropriate amount of vitamin B1 and vitamin C standard products, place them in 10mL volumetric flasks, dissolve with 10mg / mL dithiothreitol in 50% methanol aqueous solution and adjust the volume to the mark to prepare Vitamin B1 and vitamin C stock solutions with concentrations of 0.787mg / mL and 0.945mg / mL were prepared. Dissolve and transfer vitamin B1-13C4 and vitamin C-13C6 in 10mg / mL dithiothreitol in 50% methanol...

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Abstract

The invention discloses a vitamin B1 and vitamin C concentration rapid detection method based on a liquid chromatography-tandem mass spectrometry technology. The method comprises the following steps:preparing a vitamin B1 and vitamin C reference substance solution and a vitamin B1 and vitamin C isotope internal standard solution; establishing standard curves of vitamin B1 and vitamin C; collecting a chromatogram of the serum sample solution; and determining the concentrations of vitamin B1 and vitamin C in the serum sample. The detection method is simple, sensitive, rapid, accurate, high in specificity and good in repeatability, and can be used for rapid detection of vitamin B1 and vitamin C in serum.

Description

technical field [0001] The invention relates to a method for detecting water-soluble vitamins, in particular to a method for rapidly detecting the concentrations of vitamin B1 and vitamin C based on liquid chromatography tandem mass spectrometry technology. Background technique [0002] Vitamins, also known as vitamins, are nutrients that maintain life. It plays an important role in the process of human growth, development, and metabolism. Such substances cannot be synthesized in the body or the amount of synthesis is insufficient, so they must be continuously replenished from the outside world. According to the difference in structure and solubility, vitamins are divided into fat-soluble vitamins and water-soluble vitamins. Water-soluble vitamins refer to vitamins that can dissolve in water, usually as a component of coenzymes or prosthetic groups, including B vitamins and vitamin C. [0003] Chinese invention patent: 2016105698131, a method for rapid determination of vit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8679G01N2030/045
Inventor 江振作李青丁娜
Owner 合肥谱佳医学检验实验室有限公司
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