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RNA inhibitor and application thereof

An inhibitor, OLN-93 technology, applied in the field of biomedicine, can solve problems such as failure to work normally, unsatisfactory clinical efficacy, etc., and achieve the effect of alleviating activation and reproduction, far-reaching social significance, and important medical significance

Pending Publication Date: 2020-04-03
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to statistics, less than 1% of SCI patients can fully recover neurological function, and most patients need long-term hospitalization, rehabilitation, and full-time or part-time caregivers, so that they are often unable to work normally, which brings great harm to the family and society. However, the drug treatment and surgical treatment of SCI at home and abroad have not achieved satisfactory clinical curative effect.

Method used

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  • RNA inhibitor and application thereof
  • RNA inhibitor and application thereof
  • RNA inhibitor and application thereof

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Effect test

Embodiment 1

[0028] The invention provides an RNA inhibitor, which is a miRNA-17-5p inhibitor and has a miRNA-17-5p inhibitory sequence.

[0029] The miRNA-17-5p inhibitory sequence is:

[0030] 5’-3”CUACCUGCACUGUAAGCACUUUG.

Embodiment 2

[0032] The invention provides an application of miRNA-17-5p inhibitor in inhibiting the activation and reproduction of astrocytes after spinal cord injury.

[0033] A method for reducing the activation of astrocytes after spinal cord injury using miRNA-17-5p inhibitors, comprising the steps of:

[0034] (1) Firstly, the miRNA-17-5p inhibitory sequence is given to prepare the miRNA-17-5p inhibitor;

[0035] The miRNA-17-5p inhibitory sequence is:

[0036] 5’-3”CUACCUGCACUGUAAGCACUUUG.

[0037] (2) Collect exosomes secreted by oligodendrocytes;

[0038] (3) Co-incubate the collected exosomes with the damaged astrocytes to realize the uptake of exosomes by the astrocytes and reduce the excessive activation of the damaged astrocytes; the specific operation method is: Astrocytes at 2 x 10 3 Each well was inoculated in a 96-well plate pre-coated with poly-lysine and placed at 37°C in 5% CO 2 Cultured in the incubator for 48 hours, after the cells entered the logarithmic growth ...

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Abstract

The invention provides an RNA inhibitor and application thereof. The RNA inhibitor is an miRNA-17-5p inhibitor and has an miRNA-17-5p inhibition sequence. The miRNA-17-5p inhibitor can be used for inhibiting activation and proliferation of astrocytes after spinal cord injuries. According to the RNA inhibitor, oligodendrocytes after the spinal cord injuries are applied to generating a large quantity of exosomes carrying miR-17-5p, the exosomes can be taken in by the astrocytes, and thus activation characteristics of the astrocytes are promoted; and an antagonist of the miR-17-5p is designed, thus, a purpose of reducing astrocyte excessive proliferation is achieved, and an effective therapeutic target is provided for treating the spinal cord injuries.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to an RNA inhibitor and application thereof. Background technique [0002] Spinal cord injury (SCI) is a serious central nervous system injury disease, involving millions of patients all over the world, and their quality of life and work are seriously affected. According to statistics, less than 1% of SCI patients can fully recover neurological function, and most patients need long-term hospitalization, rehabilitation, and full-time or part-time caregivers, so that they are often unable to work normally, which brings great harm to the family and society. However, the drug therapy and surgical treatment of SCI at home and abroad have not achieved satisfactory clinical efficacy. Therefore, it is of great significance to study the mechanism and treatment of SCI. Astrocytes are the most numerous and abundant cells in the central nervous system (Central Nervous System, CNS). Ast...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113A61P25/00
CPCC12N15/113A61P25/00C12N2310/113
Inventor 陈颖刘永华王婧杨莹曹淑艳王晓冬
Owner NANTONG UNIVERSITY
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