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A fluorescent probe for copper (ii) ion detection and its application

An ion detection and fluorescent probe technology, applied in the field of fluorescence detection, can solve the problems of expensive instruments, high cost, complicated operation process, etc.

Active Publication Date: 2021-09-10
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods have high sensitivity and specificity, the instruments used are expensive, costly, and complicated to operate.

Method used

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  • A fluorescent probe for copper (ii) ion detection and its application
  • A fluorescent probe for copper (ii) ion detection and its application
  • A fluorescent probe for copper (ii) ion detection and its application

Examples

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Effect test

Embodiment 1

[0030] 1, the preparation method of FAM-DNA fluorescent probe solution, the steps are as follows:

[0031] Prepare a Tris-HCl buffer solution with a pH of 6.0 and a concentration of 10 mM with deionized water; centrifuge the FAM-DNA fluorescent probe with a centrifuge, add Tris-HCl buffer (10 mM, pH 6.0), and mix on a shaker After 5 minutes, prepare 10 μM FAM-DNA, dilute to 100 nM, and put in a 4°C refrigerator for later use.

[0032] 2, the preparation method of copper ion solution, the steps are as follows:

[0033] Weigh 0.0034g of CuCl with an analytical balance 2 2H 2 O, add the prepared Tris-HCl buffer solution (10 mM, pH 6.0) to dissolve, dilute to a series of concentrations of 200nM, 1.6μM, 4μM, 8μM, 16μM, 30μM, 40μM, 50μM, 60μM, 100μM, and put it in a 4℃ refrigerator stand-by.

[0034] 3. The detection steps of copper ion solution are as follows:

[0035] Take an equal volume of FAM-DNA fluorescent probe solution, add copper ion solutions with final concentration...

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Abstract

The invention discloses a fluorescent probe for detecting copper (II) ions, which is composed of DNA modified with a FAM fluorescent group, and the DNA sequence is 5'-AAAAAAAAGCGC-3'. The fluorescent probe has high selective recognition for copper (II) ions and high sensitivity, can realize the detection of copper (II) ions in a complex metal ion solution system, and the fluorescent probe is simple in design and low in cost, which is favorable for popularization and application.

Description

technical field [0001] The present invention relates to a kind of fluorescent probe, relate in particular to a kind of FAM-DNA fluorescent probe about copper (II) ion detection, also relate to a kind of FAM-DNA fluorescent probe based on copper (II) ion and make it quench The method for detecting copper ions through the change of fluorescence signal belongs to the field of fluorescence detection. [0002] technical background [0003] Metal ions can not only maintain the structure of biomolecules, but also participate in various life processes. Copper ions play an important role in biological systems, and their concentration directly affects people's health. In the environment, especially in water quality, copper ions have been identified as an environmental pollutant, so accurate detection of divalent copper ions is of great significance in the fields of environmental and biological analysis. [0004] At present, the methods for detecting copper ions mainly include inductiv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6816G01N21/64C12N15/11
CPCC12Q1/6816G01N21/6428G01N2021/6432C12Q2563/107C12Q2563/137
Inventor 向娟沈宝钗
Owner CENT SOUTH UNIV
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