Bacillus velezensis and application thereof in prevention and treatment of porcine diarrhea

A technology of Bacillus Velez and Bacillus, applied in the field of microbiology, can solve the problem that no one isolates the strain of Bacillus Velez, and achieve the effects of not being easy to die, reducing the rate of diarrhea, and reducing diarrhea

Active Publication Date: 2020-04-10
YUNNAN ANIMAL SCI & VETERINARY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past two years, a few studies have found that Bacillus Velez can also be isolated from the intestines of some specific animals, such as the Chinese patent "Bacillus Velez and Its Application as an Inhibitor of Aquatic Pathogens" with publication number CN108676756B ", the Bacillus Velez is isolated from the intestinal tract of tilapia; t

Method used

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  • Bacillus velezensis and application thereof in prevention and treatment of porcine diarrhea
  • Bacillus velezensis and application thereof in prevention and treatment of porcine diarrhea
  • Bacillus velezensis and application thereof in prevention and treatment of porcine diarrhea

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0037] Example 1: Separation and identification of Bacillus sp. strain LL2018013

[0038] Step 1) Screening of Bacillus strains

[0039] Take the contents of the small intestine, cecum and large intestine of Diqing Tibetan pigs in sterilized normal saline (w / v=1%), shake thoroughly, and bathe in 80℃ water for 10 minutes. After gradient dilution, plate into LB solid medium for cultivation. After culturing at 35°C for 12 hours, a single colony with typical characteristics of Bacillus was picked and purified and cultured to obtain Bacillus.

[0040] Step 2) Screening of antagonistic strains

[0041] Use the bacillus obtained in step 1) to prepare the bacterial solution to be tested, use Escherichia coli K88, Salmonella typhimurium and Staphylococcus aureus as indicator bacteria, prepare a suspension bacterial solution with 0.9% saline and adjust the concentration, and add it to the agar In the medium, the preparation contains about 10 indicator bacteria 5 CFU / mL agar plate, after coolin...

Example Embodiment

[0058] Example 2 Feeding experiment of weaned piglets

[0059] Preparation of fresh A1 bacterial solution: the above-mentioned Bacillus Velezia strain LL2018013 was inoculated into LB liquid medium at a volume percentage of 0.1%, and cultured at 30-35°C for 20-24 hours to obtain A1 fresh bacterial solution.

[0060] Feeding experiment: 40 7-day weaned piglets of Diqing Tibetan pigs were selected, and they were randomly divided into two groups with half male and half female. The experimental group was fed milk replacer + A1 fresh bacterial solution (10 8 CFU / L), the control group was fed milk replacer. The trial period was 21 days, of which the pre-test was 7 days and the positive trial was 14 days. Morbidity and mortality were observed.

[0061] Diarrhea rate = number of diarrhea heads / (total number of experimental piglets x number of positive test days) x 100%

[0062] Table 3 Diarrhea and survival rate of weaned piglets

[0063]

Example Embodiment

[0064] Example 3 Test on the abundance of intestinal flora in growing pigs

[0065] 1) Prepare A1 dry bacteria powder:

[0066] The Velez Bacillus strain LL2018013 described in Example 1 was inoculated into LB liquid medium at a volume percentage of 0.1%, cultured at 35°C for 20 hours, and then centrifuged at 10,000 rpm to obtain the bacteria; add appropriate amount of purified water to the above bacteria and mix well Mud-like, after mixing with the protective agent, add an appropriate amount of purified water, spray drying with the embedding material to obtain dry bacteria powder;

[0067] Wherein, the protective agent components and proportions are: skimmed milk powder 15%, glycerol 5%, R3-type resistant starch 8%, vitamin E 2%, maltodextrin 3%, agar powder 3%, and the rest are potato resistant Starch; the embedding material is potato resistant starch, pectin, sodium hydroxymethyl cellulose, carrageenan, and the embedding material is formulated into a 5% aqueous solution according...

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Abstract

The invention relates to bacillus velezensis and an application thereof in prevention and treatment of porcine diarrhea. The invention provides a bacillus velezensis strain LL2018013. The bacillus velezensis is added into a weaned piglet milk replacer, wherein the addition amount is 107 to 109 CFU/L; and the bacillus velezensis is added into a feed for pigs at growth periods, wherein the additionamount is 106 to 108 CFU/g. The bacillus velezensis provided by the invention can improve the diversity of pig intestinal flora, reduces inflammatory factors through regulation of the flora abundance,promotes nutrient digestion and absorption, improves oxidation resistance of a body, improves immunity, increases the resisting capability to viruses, reduces generation of diarrhea, can inhibit common pig intestinal pathogens like escherichia coli K88, salmonella typhimurium and staphylococcus aureus, reduces the rate of diarrhea, improves the daily gain of pigs, can inhibit molds in a feed, improves the metabolic efficiency of the body to lipids, improves digestion and absorption of the pigs to feed nutritional ingredients, and has the trend of reducing chemical residues in excrement at thesame time.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a strain of Bacillus velei and its application in the prevention and treatment of swine diarrhea. Background technique [0002] At present, with the improvement of the level of animal husbandry in my country, the livestock and poultry breeding industry has played an important role in my country's agricultural economic industry. With the development of the industry, antibiotics, hormones and other drugs have brought contradictory status quo. On the one hand, they bring rich production benefits, such as: promoting growth, increasing feed remuneration, and reducing morbidity; on the other hand, they destroy the balance of flora and inhibit Immune response, antibiotic residues, environmental pollution caused by antibiotics in animal excrement, deterioration of soil environment, and a series of reactions. [0003] Pigs are the most widely raised livestock in my country, and pig diar...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04A23K10/18A23K50/60A23K50/30C12R1/07
CPCA23K10/18A23K50/30A23K50/60C12N1/04C12N1/20C12N1/205C12R2001/07
Inventor 刘韶娜赵彦光张斌相德才赵智勇
Owner YUNNAN ANIMAL SCI & VETERINARY INST
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