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A kind of deodorizing bacterium agent and application thereof for removing bad smell in dry toilet

A deodorizing bacterial agent and odor technology, which are applied to the deodorizing bacterial agent for removing odor in dry toilets and its application field, can solve problems such as bad odor in dry toilets, and achieve the effects of removing odor, inhibiting growth and low cost.

Active Publication Date: 2020-10-16
QINGDAO SHANGDE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the problem that the prior art cannot quickly and fundamentally solve the stench of dry toilets, the present invention provides a deodorant bacterial agent for removing odor in dry toilets and its application. The deodorant bacterial agent is simple to operate and low in cost , green and environmental protection, and will not cause secondary pollution to the environment

Method used

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  • A kind of deodorizing bacterium agent and application thereof for removing bad smell in dry toilet
  • A kind of deodorizing bacterium agent and application thereof for removing bad smell in dry toilet
  • A kind of deodorizing bacterium agent and application thereof for removing bad smell in dry toilet

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Isolation, screening and identification of Lactobacillus pasteri GBW-HB1903

[0036] 1. Screening of Lactobacillus pasteri GBW-HB1903

[0037] Municipal domestic sewage was used as the sample, and after gradient dilution, the MRS medium containing calcium carbonate was used to pour culture to screen the colony with obvious "calcium dissolution circle". After multiple purifications, a single colony was obtained, named GBW-HB1903, and preserved.

[0038] Such as figure 1 As shown, the colony of the strain GBW-HB1903 on the MRS plate is round, milky white, 1-2 μm in diameter, smooth, moist and shiny, slightly raised in the middle, opaque, with neat edges and no halos. The strain GBW-HB1903 can grow and reproduce normally in the temperature range of 10-45°C, and its optimum growth temperature is 25-35°C; it can grow in the range of pH 6-9, and the optimum growth pH value is 6.5 ~7.5; it can grow normally when the dissolved oxygen content is less than 0.5mg / L or greater...

Embodiment 2

[0057] The preparation method of the deodorant bacterium agent that removes bad smell in dry toilet according to the present invention is as follows:

[0058] 1. Lactobacillus pasterneri, Clostridium butyricum and Bacillus psychrophilus were respectively activated and cultured in the corresponding culture medium.

[0059] Among them, Lactobacillus pasteri culture medium is: glucose 50-100g, soybean peptone 5-15g, yeast extract 1-5g, K 2 HPO 4 1~4g, triammonium citrate 1.5~3g, MgSO 4 ·7H 2 O0.5~1g, sodium acetate 1~5g, Tween 801~3ml, CaCO 3 1~7g, MnSO 4 4H 2 O0.15~0.5g, distilled water 1000ml, pH6.0~6.5, culture time 16~24 hours, culture temperature 25~35℃.

[0060] Clostridium butyricum medium: glucose 30-70g, soybean peptone 5-20g, yeast extract 2-5g, sodium acetate 5-10g, sodium chloride 1-3g, MgSO 4 0.01~0.05g, K 2 HPO 4 0.5~3g, Na 2 CO 3 5-15g, pH 6.5-7.5, culture time 24-48 hours, culture temperature 25-35°C.

[0061] Psychrotrophic bacillus culture medium: gl...

Embodiment 3

[0067] Collect excrement from dry toilets of a vocational and technical school in Qingdao, and divide it into 9 identical plastic cylindrical barrels with a diameter of 45cm and a height of 150cm. Initially, each barrel is filled with 5kg. An additional 0.5 kg of feces was added, which were divided into 3 test groups, and each group was treated 3 times in parallel, namely the CK group without any treatment, the A group added with inactivated deodorant bacteria, and the normal deodorant bacteria Group B. The deodorant bacteria agent should be diluted 5 to 10 times before use, and then poured directly into the bedpan or evenly sprayed on the surface of the feces. For ~7 days, you need to use it once a day according to the usage method. After 7 days, you can use it 1-2 times a week according to the odor. The whole test period is 15 days; during the test, the test indicators are detected every other day and artificial sensory records are made, wherein the test detection indicator...

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Abstract

The invention provides a deodorizing microbial inoculum for removing odor in pit toilets and application of the deodorizing microbial inoculum. The deodorizing microbial inoculum is prepared by expanding culture of a mixed thallus seed solution and comprises lactobacillus paratyphi with the preservation number of CGMCC NO.18391, clostridium butyricum with the preservation number of CGMCC NO.14499and bacillus psychrophilus with the preservation number of CGMCC NO.18393. The deodorizing microbial inoculum is mainly applied to rural ecological toilets, schools, scenic spots, public pit toilets and other pool bodies or equipment for storing and treating excrement, is wide in application scenarios, in addition, the deodorizing microbial inoculum not only can remarkably reduce odor factors in the excrement, but also can quickly degrade residual organic matters; therefore, the odor in the environment is fundamentally and remarkably removed, the air quality is improved, the human sensory feeling effect is improved, and the characteristics of controllable production, stable product quality, low cost, thorough deodorization, environmental protection and no secondary pollution are achieved.

Description

technical field [0001] The invention belongs to the biological technical field of a dry toilet deodorization treatment method, in particular to a deodorizing bacteria agent for removing odor in dry toilets and an application thereof. Background technique [0002] Stench refers to the general term for all gases that stimulate the olfactory organs to cause people's unpleasantness and damage the human living environment. Stink not only affects people's mood, but also makes people feel irritable, depressed and other bad feelings. If they are in a high-concentration stench environment or in a low-concentration stench environment for a long time, the human body will also be seriously injured. In addition, the stench gas can also affect animals and plants. Therefore, research on deodorization technology has become an important link in environmental governance engineering. The generation of malodorous gas is generally attributed to the anaerobic decomposition of organic matter. The...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B01D53/84B01D53/52B01D53/58C12R1/225C12R1/07C12R1/145
CPCB01D53/52B01D53/58B01D53/84C12N1/20Y02A50/20
Inventor 袁绍辉王熙涛赵金超孙志刚
Owner QINGDAO SHANGDE BIOTECH
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