Method for preparing ovarian cancer organoid
A technology for ovarian cancer cells and cancers, which is applied in the field of biomedicine and can solve the problem of difficult tissue blocks.
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[0039] Example
[0040] 1. Feeder cell preparation
[0041] The human endometrial stem cells (Beijing Crespo, CE20016) were plated at a density of about 5000 cells per well in a 96-well plate containing 50μl of Cosmo thermosensitive hydrogel per well, and stored in Stemedia TM WIT-I TM Cultivate 24h in the medium at 37℃. After the culture, the whole 96-well plate with human endometrial stem cells is placed in an X-ray irradiator for irradiation. The irradiation dose is 80KV and the irradiation time is 30min. After the irradiation, the human uterus Intimal stem cells are used as feeder cells for later use.
[0042] 2. Preservation of puncture tissue
[0043] Store fresh ovarian cancer puncture tissue samples in Stemedia containing 150U / ml penicillin and 150ug / ml streptomycin TM WIT-I TM In the culture medium, and transported to the laboratory cold chain at 4°C within 48 hours.
[0044] 3. Obtain ovarian cancer cells
[0045] Cut the puncture tissue sample of ovarian cancer into sm...
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