Method for killing salmonellas through photodynamics

A Salmonella, photodynamic technology, applied in the direction of egg preservation, egg preservation through radiation/electrical treatment, food science, etc., to achieve the effect of cost-free pollution, significant effect, and simple operation

Pending Publication Date: 2020-04-28
SHANGHAI OCEAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Therefore, to use photodynamic sterilization technology to kill Gram-negative bacteria, especially Salmonella in food, it is urgent to adopt photosensitizers that can be applied in food, such as riboflavin, and riboflavin is not used in the prior art. Photodynamic approach to kill Salmonella as a photosensitizer

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  • Method for killing salmonellas through photodynamics
  • Method for killing salmonellas through photodynamics
  • Method for killing salmonellas through photodynamics

Examples

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preparation example Construction

[0044] Preparation of Salmonella Bacteria Liquid

[0045] The Salmonella species used in the examples are Salmonella typhi CICC 21484 and Salmonella enteritidis CMCC 50041, respectively. Among them, Salmonella typhimurium CICC 21484 was purchased from China Center of Industrial Culture Collection (CICC); Salmonella enteritidis CMCC 50041 was purchased from China Medical Culture Collection (CMCC) .

[0046] Preparation method of Salmonella bacterial liquid: take the standard strains CICC 21484 and CMCC50041 stored in glycerol tubes stored at -80°C and inoculate them by streaking on bismuth sulfite agar plates, and culture them statically at 37°C for 24-48 hours. Single colonies were picked and placed in 9mL TSB test tubes, and cultured in a shaker at 37°C with a rotation speed of 180r / min for 13h to obtain the bacterial culture solution in the initial stage of stability. Mix the same amount of two Salmonella culture solutions in a centrifuge tube, centrifuge for 5 minutes (4°...

Embodiment 1

[0063] Effect of different riboflavin concentrations on the effect of photodynamic killing of Salmonella

[0064] The experiment was carried out according to the above method of photodynamic treatment of Salmonella, wherein the concentration of riboflavin solution in the mixed system of the sample to be treated was 0 μmol / L, 50 μmol / L, 100 μmol / L, 150 μmol / L, 200 μmol / L, 250 μmol / L L, 300 μmol / L; dark incubation time is 40 minutes; LED blue light source irradiation time is 30 minutes. figure 2 The inactivation of Salmonella after treatment with different concentrations of riboflavin is shown in .

[0065] The initial inoculum of Salmonella in the system was about 4.5×10 6 CFU / mL, when the incubation time is 40min, the light time is 30min, when the riboflavin concentration is 100, 150, and 200μmol / L, the amount of Salmonella can be reduced by 1.12, 6.14, and 6.21Log CFU / mL, of which riboflavin When the concentration was 150μmol / L and 200μmol / L, the lethality rate of Salmonel...

Embodiment 2

[0068] Effect of different incubation times on the effect of photodynamic killing of Salmonella

[0069] The experiment was carried out according to the above method of photodynamic treatment of Salmonella, wherein: the concentration of riboflavin in the mixed system was 150 μmol / L, the incubation time was 0 min, 20 min, 40 min, and 60 min, respectively; the irradiation time of the LED blue light source was 30 min. Such as image 3 As shown, as the incubation time increases, the killing effect on Salmonella also increases, and when the incubation time is 40min, the lethality rate of Salmonella is 99.99993%. The incubation time is too long will also affect the bactericidal effect. Therefore, it is necessary to choose an appropriate incubation time to have a better killing effect on Salmonella.

[0070] Two groups of control experiments were set up according to the above-mentioned method of photodynamic treatment of Salmonella. The irradiation time of one group of LED blue li...

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Abstract

The invention discloses a method for killing salmonellas through photodynamics. The method is a method for killing salmonellas by taking riboflavin as a photosensitizer and adopting a blue light source and photodynamics, and belongs to the technical field of sterilization for killing food-borne pathogenic bacteria, salmonellas. The photosensitizer adopted by the invention is one of vitamins necessary for human bodies. The riboflavin belongs to a food-grade photosensitizer, is safe and non-toxic, and has a remarkable salmonella killing effect. Moreover, the method can control the risk of salmonellosis, is short in treatment time and simple and convenient to operate, can thoroughly kill the salmonellas, and has certain control and prevention effects. The invention provides the method for effectively killing the salmonellas in foods, and the method is low in cost, simple to operate and wide in application, and well promotes the development of a food sterilization technology.

Description

technical field [0001] The invention relates to a sterilizing technology for food-borne pathogenic bacteria Salmonella, in particular to a photodynamic method for killing Salmonella. Background technique [0002] With the frequent occurrence of vicious food safety incidents around the world in recent years, food contamination and foodborne diseases constitute a huge worldwide public health problem, and have also become important factors hindering the development of international food trade. Salmonella is the main pathogen of foodborne diseases in the world. It is aggressive and can release endotoxin with strong heat resistance after cracking. In recent years, the food poisoning cases caused by Salmonella have been in the forefront of various poisoning incidents. Moreover, Salmonella has strong resistance to different external environments and can survive for several months in water, milk and meat products. At the same time, Salmonella cells have cold shock protein (CspH), wh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L5/20
CPCA23L5/20A23L5/27A23L3/28A23L3/3544A23L3/26A23B5/015A23B5/14
Inventor 赵勇李慧慧王敬敬刘海泉陈博文黄嘉明
Owner SHANGHAI OCEAN UNIV
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