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Liquiritin and glycyrrhizic acid components enriched in liquorice extract and preparation method of liquiritin and glycyrrhizic acid components

A technology of licorice extract and liquiritin, which is applied in the field of enrichment of liquiritin and glycyrrhizic acid components in licorice extract and its preparation, can solve the problems of low yield of preparation process, low content of target components, high production cost, etc. Achieve the effects of simple operation, saving the cost of medicinal materials, improving quality and yield

Pending Publication Date: 2020-04-28
TAIZHOU GUOKEHUAWU BIOMEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The present invention mainly provides a method for enriching liquiritin and glycyrrhizic acid components in licorice extract and its preparation method, which is used to solve the problem of low yield in the extraction and preparation process of licorice medicinal materials, low content of target components in purified products, and the use of a large amount of organic solvents. Cause environmental pollution and high production costs

Method used

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  • Liquiritin and glycyrrhizic acid components enriched in liquorice extract and preparation method of liquiritin and glycyrrhizic acid components
  • Liquiritin and glycyrrhizic acid components enriched in liquorice extract and preparation method of liquiritin and glycyrrhizic acid components
  • Liquiritin and glycyrrhizic acid components enriched in liquorice extract and preparation method of liquiritin and glycyrrhizic acid components

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Effect test

Embodiment 1

[0027] The present invention proposes a preparation method for enriching glycyrrhizin and glycyrrhizic acid components in licorice extract, the specific steps are as follows:

[0028] Weigh 1Kg of pretreated licorice, add 10 L of water, put it in a decoction machine and boil it (120°C) for 1 hour, filter it with gauze, add 10 L of water to the dregs of the medicine, put it into the medicine decoction machine again and cook it (120°C) for 0.5 hour, filter it with gauze , combined the filtrates, concentrated by rotary evaporation, and dried under reduced pressure to obtain licorice extract powder. The sample that the licorice extract powder dissolves is carried out HPLC analysis, and chromatographic analysis result is as follows: figure 1 shown.

[0029] Connect a C18 reversed-phase chromatographic column (filler weight 300 g, particle size 60 μm) to a high-pressure liquid phase system with a design flow rate of 60 ml / min (190 cm / h), and wash the column 3BV with 5% ethanol equi...

Embodiment 2

[0031] The present invention proposes a preparation method for enriching glycyrrhizin and glycyrrhizic acid components in licorice extract, the specific steps are as follows:

[0032] Weigh 1Kg of the pretreated licorice, add 20 L of water, put it in a decoction machine and boil it (120°C) for 0.8 h, filter it with gauze, add 10 L of water to the dregs, put it into the decoction machine again and boil it (120°C) for 0.4 h, Filtrate with gauze, combine filtrates, concentrate by rotary evaporation, and then spray dry to obtain licorice extract powder.

[0033]Connect a C18 reverse-phase chromatographic column (filler weight 300 g, particle size 30 μm) to a high-pressure liquid phase system with a design flow rate of 60 ml / min (190 cm / h), and use 10% ethanol equilibration buffer to wash the chromatographic column 2BV, Purify 80 g of licorice extract powder dissolved in equilibration buffer (use a 0.22 μm filter membrane to remove particulate insoluble matter) through the system p...

Embodiment 3

[0035] The present invention proposes a preparation method for enriching glycyrrhizin and glycyrrhizic acid components in licorice extract, the specific steps are as follows:

[0036] Weigh 1 Kg of the pretreated licorice, add 20 L of water, put it in a decoction machine and boil it (120°C) for 1 h, filter it through gauze, add 10 L of water to the dregs, put it into the decoction machine again and boil it (120°C) for 0.5 h , filtered through gauze, combined filtrates, concentrated by rotary evaporation, and then spray-dried to obtain licorice extract powder.

[0037] Connect a C18 reverse-phase chromatographic column (filler weight 300 g, particle size 10 μm) to a high-pressure liquid phase system with a design flow rate of 50 ml / min (150 cm / h), and wash the column 2BV with 5% ethanol equilibration buffer, 60 g of licorice extract powder dissolved in equilibration buffer (use a 0.22 μm filter membrane to remove particulate insoluble matter) was purified by flowing through the...

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Abstract

The invention relates to liquiritin and glycyrrhizic acid components enriched in a liquorice extract and a preparation method of the liquiritin and glycyrrhizic acid components, in particular to the processes of water extraction, clarification and filtration, preparative chromatography enrichment and concentration and drying. The method comprises the following steps: preparing a liquorice extractsolution from a liquorice medicinal material as a raw material through water extraction, concentration drying and powder dissolution, clarifying a crude extract by adopting a membrane filtration technology, and carrying out enrichment and purification by utilizing a high-pressure preparation system through reversed-phase preparative chromatography. The contents of liquiritin and glycyrrhizic acidcomponents in a sample prepared by purification are high, the production process is simple to operate and high in efficiency, and the process is easy to amplify. The components enriched and prepared by the method disclosed by the invention can be widely applied to the industries of medicines, health products and the like, and have wide market prospects and good economic benefits.

Description

technical field [0001] The invention relates to the technical field of Chinese herbal medicine processing, in particular to a method for enriching liquiritin and glycyrrhizic acid components in licorice extract and the preparation method thereof. Background technique [0002] Licorice is the dried root and rhizome of Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L., a leguminous plant. Licorice is widely distributed in my country, mainly produced in Xinjiang, Inner Mongolia, Gansu, Shanxi, Shaanxi, Northeast and other places. Often used as an adjuvant drug in a variety of traditional Chinese medicine compound, sweet in taste, flat in nature, homecoming to the heart, lung, spleen, and stomach meridian, has the effects of invigorating the spleen and replenishing qi, clearing away heat and detoxification, eliminating phlegm and relieving cough, relieving spasm and relieving pain, and harmonizing the effects of various medicines. Modern pharmacolo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/26C07H1/08C07J63/00C07H15/256
CPCC07H15/26C07H1/08C07J63/00C07H15/256
Inventor 梁鑫淼叶贤龙郭志谋王超然顾佳华
Owner TAIZHOU GUOKEHUAWU BIOMEDICAL TECH CO LTD
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