Integrated wafer and method for sperm sorting, egg cultivation and in-vitro fertilization

Abstract

The invention provides an integrated wafer. The integrated wafer comprises a sperm sorting system, an egg cultivating and sperm-egg binding system and a liquid perfusion system. The sperm sorting system comprises an inlet part, an outlet part, a main flow channel and a branch flow channel. The main flow channel has a width that increases from the inlet portion toward the outlet portion. The branchflow channel is communicated with the main flow channel. The ovum cultivating and sperm-egg binding system is communicated with the branch flow channel and comprises a first through hole part and a second through hole part. The aperture of the first through hole is larger than the average diameter of the eggs in order to contain the eggs to be bound with the sperms. The second through hole part is located below the first through hole part and has an aperture smaller than that of the egg. The sorted sperms are guided into the egg cultivating and sperm-egg binding system through the branch flowchannel so as to be combined with the eggs in the first through hole. The liquid perfusion system is located below the second through hole part and exchanges liquid with the cultivating and binding system through the second through hole part. The process is mild, sperm and eggs can be prevented from being damaged, and the survival rate and the binding rate of the sperms and eggs are greatly increased.

Description

technical field [0001] The present invention relates to a system and method, and more particularly to an integrated chip and method for sperm sorting, egg cultivation, in vitro fertilization. Background technique [0002] According to statistics, the infertility rate among couples of childbearing age in Taiwan is about 10-15%. Faced with such a high rate of infertility, in vitro fertilization (IVF), commonly known as "test-tube baby" ) has a successful pregnancy rate of only 33%. Such a low success rate is mostly due to the fact that the sample handling method is not gentle enough or due to human error during the implementation of traditional IVF technology, which leads to a significant decline in the health of the sample or even death. If it can be developed A system can handle specimens in a more gentle manner, and it is believed that the success rate of in vitro fertilization technology can be significantly improved. Contents of the invention [0003] The present inven...

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Problems solved by technology

[0002] According to statistics, the infertility rate among couples of childbearing age in Taiwan is about 10-15%. Faced with such a high rate of infertility, in vitro fertilization (IVF), commonly known as "test-tube baby" ) has a successful pregnancy rate of only 33%. Such a low success rate is mostly due to the fact that the sample handling method is not gentle enough or due to human error during the implementation of traditional IVF technology, which leads to a significant decline in the health of the sample or even death. If it can be developed A system can handle specimens in a more gentle way, and it is believed that the success rate of in vitro fertilization technology can be significantly improved

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