Compound capable of increasing skin anti-wrinkle function, and use thereof in preparing cosmetic
A technology of anti-wrinkle cosmetics and compounds, applied in the field of biomedicine, can solve the problems that the improvement effect needs to be further improved, and achieve good market prospects, low cytotoxicity, and easy preparation
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Embodiment 1
[0022] Example 1 Preparation of coupling compound
[0023] Preparation of polypeptide-compound conjugate The compound of formula (II) of the present invention was synthesized according to the compound synthesis route in step A3 of Example A3 in CN201480017060.2. Then add 1mmol formula (II) compound, 1mmol O-benzotriazole-N,N,N',N'-tetramethylpirouentetrafluoroborate (TBTU), 5mlDMF, nitrogen protection in 50m reaction bottle, Add 0.5mmol) N,N-diisopropylhexylamine (DIEA) dropwise, stir at 25°C for 1.5h, add 0.1g cell activity-promoting peptide (sequence VNHNYDFPWPDSEGKPPPHWLAKSPQK), stir at 36°C for 3.5h, and stop reaction. After confirming the correct molecular weight using MS-IT-TOF, the crude product was purified using HPLC to obtain a purified product with a purity of 99.96%. The structural formula of the conjugated product obtained through structural analysis is shown in formula (I): .
Embodiment 2
[0024] Example 2 Compound Toxicity Detection
[0025] (1) Cell culture
[0026] At 37°C, 5% CO 2 Human fibroblasts were prepared by culturing in DMEM medium containing 100 U / ml penicillin / streptomycin and 10% FBS in an incubator.
[0027] (2) Preparation of medium containing the compound of formula (I)
[0028] Mix DMEM powder (GIBCO, New York, USA) in the compound of Example 1, then add antibiotics (penicillin / streptomycin of 100U / ml) and 10% FBS to prepare the medium containing 5% formula (I) compound As well as the culture medium containing 50% of the compound of formula (I), the culture medium without the compound was used as a blank control, and the culture medium added with the compound of formula (II) was used as a positive control, and the concentrations were also 5% and 50% respectively.
[0029] (3) Cytotoxicity test of compounds
[0030] The human fibroblasts prepared in step (1) were added to a 24-well plate and cultured for 24 hours, and then the medium was re...
Embodiment 3
[0032] Example 3 Detection of COL1 protein expression by Western blotting electrophoresis
[0033]Human skin fibroblasts (HFF-1) were cultured in DMEM medium containing 15% fetal calf serum to the logarithmic phase of growth, digested with 0.25% trypsin, and seeded in 6-well plates at 105 / ml, each well 2ml. Cultivate the cells overnight in a CO2 incubator at 37°C to allow the cells to adhere to the wall. When the cell density reaches about 80%, the medium is aspirated and washed with phosphate buffered saline (PBS: 0.8g NaCl; 0.2g KCl; 2.9g Na2HPO4. 12H2O; 0.27g KH2PO4 was dissolved in 800ml deionized water, stirred to dissolve, and the volume was adjusted to 1L, and the pH was adjusted to 7.4 with concentrated HCl, and autoclaved) to wash the cells 2-3 times. Add the compound sample with a final concentration of 5% formula (I), add the compound sample with a final concentration of 5% formula (II) as a control, add 2% serum DMEM to the blank and incubate for 24 hours. Then d...
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