Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Formulation for promoting targeted pollination of almond tree crops in honey bees

An almond tree and preparation technology, which is applied in the directions of animal repellents, plant growth regulators, biocides, etc., can solve the problems that cannot be used to promote pollination of almond tree crops.

Active Publication Date: 2020-05-01
国家科学与技术研究理事会 +1
View PDF13 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the specificity for sunflower crops, the composition cannot be used to promote pollination of almond tree crops

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Formulation for promoting targeted pollination of almond tree crops in honey bees
  • Formulation for promoting targeted pollination of almond tree crops in honey bees
  • Formulation for promoting targeted pollination of almond tree crops in honey bees

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1: Identification and Quantification of Volatile Compounds in Almond Flowers

[0060] To identify and quantify the volatile compounds present in almond blossoms, the following protocol was used:

[0061] Choose an almond tree branch that has 20-30 flowers in the following stages: "buds" and "day-old flowers." Using a polyethylene bag, the branch was mixed with exposed SPME fibers (solid phase microextraction) (PDMS type, ) together to collect the volatiles present in the flowers for a duration of 2 hours. Then, the fibers were desorbed in a Shimadzu QP-2010GC-MS device under the following conditions: splitless mode, helium as the carrier gas, injector temperature of 250 °C, and interphase temperature of 310 °C. The temperature ramp used was: 40(1)-5-200-15-300(1); Column: AT-5 (diameter=0.25mm, length=30m, membrane=0.25mm, flow rate=1ml / min ).

[0062] For later assay analysis and identification of this compound, the NIST05, NIST08 and SHIM2205 libraries ...

Embodiment 2

[0065] Embodiment 2: the preparation of preparation

[0066] Three different mixtures were prepared, each mixing 3 to 4 of the volatile compounds identified in Example 1. The composition of each mixture is:

[0067] - Mixture I: 50% limonene, 30% linalool, 10% benzaldehyde, 10% nonanal.

[0068] - Mixture II: 50% limonene, 30% linalool, 10% benzaldehyde and 10% 2-octanone.

[0069] - Mixture III (formulation according to the invention): 55% limonene, 30% linalool and 15% benzaldehyde.

[0070] In all cases, pure compounds with a purity greater than 95% were used Solutions were prepared by mixing the compounds without solvent at room temperature. Once prepared, the mixtures were used immediately in the experiment and stored at 2°C-8°C until the end of the experiment (4 days).

Embodiment 3

[0071] Example 3: Evaluation of mixtures by differential olfactory conditioning

[0072] Differential olfactory conditioning was performed to assess the ability of bees to discriminate between various scents and almond native floral odors. For each scent, 32 to 46 bees were used. Each bee used in the experiment was harnessed and provided with a reward-associated scent (reward conditioned stimulus, EC+) and also with almond natural flower scent without reward (no reward condition stimulation, EC-). Both stimuli were presented to the bees via a device that delivered the scent through a continuous air stream. Filter paper (30mm x 3mm) was impregnated with each mixture (4 μl) and introduced into the syringe. On the other hand, fresh flowers from almond trees were placed in a Buchner flask (500 ml) connected to the apparatus by a cannula. This conditioning was repeated 4 times with a 15 min interval between each pair of scent presentations, in pseudo-random order. The fragra...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A formulation and a composition that comprises it for promoting the pollination of almond tree crops (Prunus dulcis) by biasing the foraging preferences of the honey bee (Apis mellifera). The formulation comprises the compounds limonene, linalool and benzaldehyde. Additionally, a method for targeting the bees' pollinizing activity towards the almond tree crops by using the formulation comprising the compounds limonene, linalool and benzaldehyde.

Description

[0001] The present disclosure relates to formulations and compounds that promote pollination of almond tree crops (Prunus dulcis) by biasing the foraging preferences of honeybees (Apismellifera). It also relates to a method of targeting almond tree crops for pollination activity of bees. Background technique [0002] In bees, odor plays an important role in their nutrient source foraging and selection. It is well known that honeybees control resource collection not only on the basis of innate foraging patterns, but also on previous experience gained in the wild as well as in the social environment of the hive (Ribbands CR (1955) The scent language of honey bees; Ann Rev Smithson Inst 368-377; von Frisch K (1967) The dance language and orientation of bees; Harvard University Press, Cambridge, MA). In this sense, olfactory memories established in the wild based on the scent of flowers and their nectar can be restored (evoke) by providing this floral scent inside beehives (Ribba...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/30A23K20/105A23K20/163A23K50/90A01N27/00A01N31/04A01N35/04
CPCA01N27/00A23K10/30A23K20/105A23K20/163A23K50/90A23K20/111A01N31/02A01N35/04A01G7/00A01K53/00A01N31/04
Inventor 沃尔特·M·法瑞纳C·埃斯特拉维斯巴卡拉F·帕洛蒂尼
Owner 国家科学与技术研究理事会
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com