Treatment of inflammatory diseases with inhibitors of c5a activity
A technology for inflammatory diseases and inhibitors, applied in the field of C5a activity inhibitors, can solve the problems of host organ immune attack, differentiation defects, self-molecules and tissues improperly recognized as foreign structures, etc.
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[0112] The present invention will now be further described. In the following paragraphs, different aspects of the invention are defined in more detail. Each aspect defined below may be combined with one or more than one other aspect unless clearly stated to the contrary. In particular, any feature indicated as preferred or advantageous may be combined with any other feature indicated as preferred or advantageous.
[0113] In a first aspect, the present invention relates to a compound for use in the treatment of a cutaneous neutrophil inflammatory disease in a subject, wherein said compound is an inhibitor of C5a activity, and wherein said cutaneous neutrophil inflammatory disease The disease is selected from the group consisting of hidradenitis suppurativa (HS); pyoderma gangrenosum (PG); PAPA (septic arthritis, PG, and acne); PASH (PG, acne, and hidradenitis suppurativa); inflammation, acne, PG, and hidradenitis suppurativa); Sweet syndrome (SS); subcorneal pustular dermato...
Embodiment
[0247] The following examples are provided to further illustrate the invention. However, the present invention is not limited thereto, and the following examples merely show the practicability of the present invention based on the above description.
[0248] 1. Method
[0249] 1.1 Preparation of zymosan A stock solution and activated plasma
[0250] Dissolve Zymosan A to 2 mg / ml in 50 ml sterile saline and boil at 100°C for 1 hour. After centrifugation, discard the supernatant and resuspend the pellet in 50 ml sterile saline. After the second centrifugation step, the pellet was resuspended in 5 ml sterile saline to obtain a 20 mg / ml stock solution. Aliquot the stock solution and store at -20 °C until use. To activate plasma, Zymosan A stock solution and 100 μl plasma were mixed and incubated at 37 °C for 30 min. After incubation, the tubes were centrifuged and the supernatant was aliquoted and stored at -20°C until use.
[0251] 1.2 CD11b assay using rhC5a or ZAP as ...
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