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A kind of carbon quantum dot fluorescent microsphere standard substance and preparation method thereof

A technology of fluorescent microspheres and carbon quantum dots, which is applied in the field of reference materials, achieving excellent stability, low cost, and simple methods

Active Publication Date: 2022-05-20
北京市计量检测科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Compared with traditional fluorescent materials and other semiconductor quantum dots, carbon quantum dots have the advantages of simple preparation process, low toxicity, good biocompatibility, environmental friendliness, and easy surface functionalization. Substances are rarely reported

Method used

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  • A kind of carbon quantum dot fluorescent microsphere standard substance and preparation method thereof
  • A kind of carbon quantum dot fluorescent microsphere standard substance and preparation method thereof
  • A kind of carbon quantum dot fluorescent microsphere standard substance and preparation method thereof

Examples

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Effect test

Embodiment 1

[0048] A carbon quantum dot fluorescent microsphere standard substance, composed of aminated polystyrene microspheres and fluorescent substances loaded on the surface of the aminated polystyrene microspheres, the fluorescent substance is carboxylated carbon quantum dots, the The carboxylated carbon quantum dots are carboxylated graphene quantum dots, and the particle size of the aminated polystyrene microspheres is 5.2 μm.

[0049] The preparation method of the carbon quantum dot fluorescent microsphere standard substance described in the present embodiment comprises the following steps:

[0050] (1) Preparation of polystyrene microspheres: 95mL of ethanol and 5mL of deionized water were added to a 250mL three-neck flask equipped with a condenser tube, 1.5g of polyvinylpyrrolidone was added and stirred, filled with nitrogen to completely dissolve the polyvinylpyrrolidone; then Add 15mL of styrene dissolved in azobisisobutyronitrile, the ratio of azobisisobutyronitrile to styre...

Embodiment 2

[0057] A carbon quantum dot fluorescent microsphere standard substance, composed of aminated polystyrene microspheres and fluorescent substances loaded on the surface of the aminated polystyrene microspheres, the fluorescent substance is carboxylated carbon quantum dots, the The carboxylated carbon quantum dots are carboxylated graphene quantum dots, and the particle size of the aminated polystyrene microspheres is 4.0 μm.

[0058] The preparation method of the carbon quantum dot fluorescent microsphere standard substance described in the present embodiment comprises the following steps:

[0059] (1) Preparation of polystyrene microspheres: 95mL of ethanol and 5mL of deionized water were added to a 250mL three-necked flask equipped with a condenser tube, 2.0g of polyvinylpyrrolidone was added and stirred, filled with nitrogen to completely dissolve the polyvinylpyrrolidone; then Add 20mL of styrene dissolved in azobisisobutyronitrile, the ratio of azobisisobutyronitrile to sty...

Embodiment 3

[0066] A carbon quantum dot fluorescent microsphere standard substance, composed of aminated polystyrene microspheres and fluorescent substances loaded on the surface of the aminated polystyrene microspheres, the fluorescent substance is carboxylated carbon quantum dots, the The carboxylated carbon quantum dots are carboxylated graphene quantum dots, and the particle size of the aminated polystyrene microspheres is 2.2 μm.

[0067] The preparation method of the carbon quantum dot fluorescent microsphere standard substance described in the present embodiment comprises the following steps:

[0068] (1) Preparation of polystyrene microspheres: 95mL of ethanol and 5mL of deionized water were added to a 250mL three-neck flask equipped with a condenser tube, 1.8g of polyvinylpyrrolidone was added and stirred, filled with nitrogen to completely dissolve the polyvinylpyrrolidone; then Add 23mL of styrene dissolved in azobisisobutyronitrile, the ratio of azobisisobutyronitrile to styre...

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Abstract

The invention provides a carbon quantum dot fluorescent microsphere standard substance, which is composed of aminated polystyrene microspheres and fluorescent substances loaded on the surface of the aminated polystyrene microspheres, and the fluorescent substance is carboxylated carbon quantum dots. The present invention also provides a method for preparing the carbon quantum dot fluorescent microsphere standard substance, comprising the steps of: (1) preparation of polystyrene microspheres; (2) preparation of nitrolated polystyrene microspheres; ( 3) Preparation of aminated polystyrene microspheres; (4) Preparation of carboxylated graphene quantum dots; (5) Preparation of standard substances of carbon quantum dot fluorescent microspheres. The carbon quantum dot fluorescent microsphere standard substance prepared by the present invention has the characteristics of not easy to fall off the fluorescence, short reaction time, long fluorescence activity, etc.; the particle size is uniform, strictly monodisperse, and has excellent stability; the method of the present invention is simple and can be operated at room temperature. The environmental impact factors are small and the cost is low, which is of great significance for the study of fluorescent standard substances.

Description

technical field [0001] The invention relates to the technical field of standard substances, in particular to a carbon quantum dot fluorescent microsphere standard substance and a preparation method thereof. Background technique [0002] Fluorescent microsphere standard substances are inseparable from flow cytometer linear calibration, optical path alignment, sensitivity, stability testing, laboratory quality control, and testing method evaluation. Flow cytometry (flow cytometry, FCM) is a single row of cells or biological particles in a fast linear flow state one by one, multi-parameter, fast qualitative, quantitative analysis or sorting technology, with fast detection speed, measurement parameters It has become a popular topic in modern scientific research due to its characteristics of large amount of collected data, comprehensive analysis, high sorting purity and flexible method. This technology integrates flow cytometry, fluorescent microspheres, laser, digital signal pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F212/08C09K11/06C09K11/65G01N15/14
CPCC09K11/06C09K11/65G01N15/14C09K2211/1425Y02B20/00
Inventor 潘一廷张国城杨振琪霍胜伟沈上圯潘素素
Owner 北京市计量检测科学研究院
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