A preparation method of highly substituted albumin methacryloyl hydrogel for cell and tissue culture

A methacryloyl water, tissue culture technology, applied in general culture methods, tissue culture, cell culture supports/coatings, etc., can solve problems such as albumin denaturation, albumin hydrogel preparation obstacles, etc. The effect of the degree of cross-linking

Active Publication Date: 2022-05-20
WENZHOU INST UNIV OF CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, albumin is easily denatured by pH and temperature, which greatly hinders the preparation of albumin hydrogels.
Therefore, there is no report on the preparation of hydrogels from albumin

Method used

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  • A preparation method of highly substituted albumin methacryloyl hydrogel for cell and tissue culture
  • A preparation method of highly substituted albumin methacryloyl hydrogel for cell and tissue culture
  • A preparation method of highly substituted albumin methacryloyl hydrogel for cell and tissue culture

Examples

Experimental program
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Effect test

Embodiment 1

[0057] In this example, 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropanol was selected as the photoinitiator, the concentration was 0.5g / 100mL, and the UV crosslinking time of 6 minutes was used to Crosslinking was performed with 10g / 100mL, 15g / 100mL and 20g / 100mL of BSA-MA with different degrees of methacrylation dissolved in PBS buffer, and the swelling and mechanical properties were affected by the concentration and the degree of methacrylation. In the process of synthesizing BSA-MA, the schematic diagram of bovine serum albumin and methacrylic anhydride reaction is as follows figure 1 shown. The specific preparation method is as follows:

[0058] 1. Dissolve BSA (V900933; Sigma-Aldrich) at 10 g / 100 mL in 200 mL of 0.25M carbonate-bicarbonate (CB) buffer (14.65 g sodium bicarbonate and 21.53 g sodium carbonate decahydrate in 1 L distilled water) at 37°C with magnetic stirring (500 rpm). After the BSA was completely dissolved, the pH of the buffer solution was adjusted to 9...

Embodiment 2

[0078] In this embodiment, the photoinitiator is 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropanol, the concentration is 0.3g / 100mL, and the crosslinking time is 15 minutes. Adjust the molar ratios of methacrylic anhydride and bovine serum albumin to 1, 2, 3, and 4, respectively, to obtain BSA-MA with different degrees of methacrylation, and adjust the concentrations of different BSA-MAs in PBS to 5 g respectively / 100mL, 23g / 100mL, 30g / 100mL, different BSA-MA hydrogels were obtained, the yield was higher, and the effect of swelling and compression was significantly different:

[0079] 1. Dissolve BSA (V900933; Sigma-Aldrich) at 10 g / 100 mL in 200 mL of 0.25M carbonate-bicarbonate (CB) buffer (14.65 g sodium bicarbonate and 21.53 g sodium carbonate decahydrate in 1 L distilled water) at 37°C with magnetic stirring (500 rpm). After the BSA was completely dissolved, the pH of the buffer solution was adjusted to 9 using 5M sodium hydroxide solution (NaOH; Sigma-Aldrich). Then dif...

Embodiment 3

[0091] In this embodiment, the photoinitiator is phenyllithium 2,4,6-trimethylbenzoylphosphinate, the concentration is 0.4 g / 100 mL, and the crosslinking time is 10 minutes. Adjust the molar ratios of methacrylic anhydride and bovine serum albumin to 1, 2, 3, and 4 respectively to obtain BSA-MA with different degrees of methacrylation, and adjust the concentrations of different BSA-MAs in PBS to 5 g / 100mL, 23g / 100mL, 30g / 100mL, different BSA-MA hydrogels were obtained, the yield was higher, and the effect of swelling and compression was significantly different:

[0092] 1. Dissolve BSA (V900933; Sigma-Aldrich) at 10 g / 100 mL in 200 mL of 0.25M carbonate-bicarbonate (CB) buffer (14.65 g sodium bicarbonate and 21.53 g sodium carbonate decahydrate in 1 L distilled water) at 37°C with magnetic stirring (500 rpm). After the BSA was completely dissolved, the pH of the buffer solution was adjusted to 9 using 5M sodium hydroxide solution (NaOH; Sigma-Aldrich). Different amounts of ...

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Abstract

The invention discloses a preparation method of high-substituted albumin methacryloyl hydrogel for cell and tissue culture, comprising: mixing bovine serum albumin and CB buffer to obtain a mixed solution, and adjusting the pH value of the mixed solution For 7-9, add methacrylic anhydride to react, and then post-process to obtain BSA-MA; dissolve BSA-MA in PBS buffer to obtain solution A; dissolve the photoinitiator in ethanol solution to obtain solution B , mix solution A and solution B to obtain a hydrogel precursor solution, which is finally obtained by casting and UV cross-linking curing. The present invention uses bovine serum albumin as a raw material to synthesize light-cured bovine serum albumin methacryloyl hydrogel for the first time, which not only ensures that the albumin will not be denatured during the preparation process, but also ensures the high substitution of acyl groups in the albumin. The prepared albumin methacryloyl hydrogel has excellent mechanical properties, water absorption, degradability and biocompatibility.

Description

technical field [0001] The invention relates to the field of hydrogel materials, in particular to a preparation method of highly substituted albumin methacryloyl hydrogel for cell and tissue culture. Background technique [0002] Hydrogels are increasingly used in drug delivery and tissue engineering applications due to their high water content and special swelling properties. Hydrogels can be divided into synthetic hydrogels and natural hydrogels according to their sources, and both of them can be used to manufacture water-swellable polymer networks with good biocompatibility to simulate the microenvironment in vivo. Synthetic polymer hydrogels can be mass-fabricated at low cost and have easily controllable mechanical properties, but they lack cellular recognition sites. Natural polymer hydrogels have good biocompatibility and biodegradability, and also have specific cell interaction sites, which are crucial for the combination of hydrogels and organisms in practical appli...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00C12N5/09C08F289/00C08F220/08C08F2/50
CPCC12N5/0068C12N5/0693C08F289/00C08F2/50C12N2533/30C08F220/08
Inventor 李培勋朱梦祥翟梦娇徐蒙蝶
Owner WENZHOU INST UNIV OF CHINESE ACAD OF SCI
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