Method for synchronously preparing functional homologous nano composition and application

A nano-composite and functional technology, applied in the field of medicine, can solve problems such as waste, and achieve the effects of avoiding waste of resources, improving stability and bioavailability, and good biocompatibility

Active Publication Date: 2020-05-15
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some functional factors such as functional alcohol-soluble proteins and polysaccharides are treated as waste in the prior art, resulting in great waste

Method used

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  • Method for synchronously preparing functional homologous nano composition and application
  • Method for synchronously preparing functional homologous nano composition and application
  • Method for synchronously preparing functional homologous nano composition and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Take 1kg of degreased and dried Coix coix powder, pass it through an 80-mesh sieve, and add 10L of distilled water to dissolve it. Adjust the pH to 3 and add 0.5% amylase. Adjust the temperature to 45°C, perform ultrasonic-assisted extraction for 120 minutes and enzymatic hydrolysis, and the ultrasonic power is 500W. Then add 20L of absolute ethanol, stir magnetically at 1000rpm for 4h, centrifuge to collect the supernatant, i.e. coix seed prolamin A solution, and the precipitate is used in subsequent processes at a centrifuge speed of 5000rpm.

[0027] (2) Add distilled water to dissolve the precipitate obtained in step (1) at a mass ratio of 1:20, raise the temperature to 95°C, keep for 120 minutes for extraction, and then separate to obtain coix seed polysaccharide B solution.

[0028] (3) Lower the temperature to 70°C, and gradually add the coix seed prolamin A solution obtained in step (1) into the coix seed polysaccharide B solution at a ratio of 1:2 (A:B,V:V...

Embodiment 2

[0030] (1) Take 1kg of degreased and dried coix powder, pass it through a 50-mesh sieve, and add 20L of distilled water to dissolve it. Adjust pH=5, add 3% amylase. The temperature was adjusted to 45°C, and ultrasonic-assisted extraction was carried out for 60 minutes for enzymatic hydrolysis, and the ultrasonic power was 500w. Then add 40L of absolute ethanol, stir magnetically at 1000rpm for 2h, and centrifuge to collect the supernatant, that is, the coix seed prolamin A solution, and the precipitate is used in the subsequent process.

[0031] (2) Add distilled water to dissolve the precipitate obtained in step (1) at a mass ratio of 1:15, adjust the pH to 7, raise the temperature to 95°C, use ultrasonic-assisted extraction for 60 minutes, and then separate to obtain coix seed polysaccharide B solution.

[0032] (3) Lower the temperature to 50°C, and gradually add the coix seed prolamin A solution obtained in step (1) into the coix seed polysaccharide B solution dropwise at...

Embodiment 3

[0034] (1) Take 1kg of degreased and dried coix powder, pass it through a 50-mesh sieve, and add 10L of distilled water to dissolve it. Adjust the pH to 3 and add 1% amylase. The temperature was adjusted to 55°C, and ultrasonic-assisted extraction was carried out for 120 minutes for enzymatic hydrolysis, and the ultrasonic power was 500w. Add 10 L of absolute ethanol, stir magnetically at 1000 rpm for 4 hours, then centrifuge at 3000 rpm, and collect the supernatant, that is, the coix seed prolamin A solution and the precipitate.

[0035] (2) Add distilled water to dissolve the precipitate obtained in step (1) at a ratio of 1:30, adjust the pH to 7, raise the temperature to 95°C, stir for 80 minutes, extract and separate to obtain coix seed polysaccharide B solution.

[0036] (3) Lower the temperature to 50°C, and gradually add the coix seed prolamin A solution obtained in step (1) into the coix seed polysaccharide B solution at a rate of 5 ml / min. After magnetically stirrin...

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Abstract

The invention discloses a method for synchronously preparing a functional homologous nano composition and application and belongs to the technical field of medicines. The method comprises the following steps: (1) filtering degreased and dried coix seed powder by using a screening net, adding distilled water, performing enzymolysis, adding absolute ethyl alcohol, performing stirring centrifugation,and collecting supernate, that is a coix seed prolamin solution A and precipitate; (2) adding distilled water into the precipitate, performing dissolution, performing heating, and performing extraction and separation so as to obtain a coix seed polysaccharide solution B; and (3) performing cooling, dripping the solution A into the solution B, performing stirring and centrifugation, so as to obtain a coix seed prolamin-polysaccharide nano composition solution C. As natural active substances are synchronously prepared, a waste, namely coix seed powder, which is generated when a coix seed esteris extracted through supercritical CO2 extraction, is adopted as a raw material, prolamin and polysaccharide in the waste are effectively utilized, resource waste is avoided, the utilization rate andthe additional value of natural products can be greatly increased, an enveloping function on instable nutrient elements can be achieved, and the enveloping rate can be up to 40%.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method and application for synchronously preparing a functional homologous nano composition. Background technique [0002] Microparticle preparations have always been a hot spot in the research of pharmacy and food industry. The use of nanoparticles to load active substances has a stable interface, and natural proteins are easy to metabolize. It is an ideal material for food and drug carriers. Edible or fully degradable natural polymer materials are ideal materials for food and drug carriers. [0003] Glyadin is a natural biopolymer with unique self-assembly properties, and is a good carrier for loading food functional components. Compared with animal prolamin, gliadin has a wide range of sources and is not easily polluted by viruses or prions. At the same time, it has biological activity, which is more conducive to the development of a new generation of nanocarri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/19A61K47/42A61K47/36A23L33/125A23L33/185A23P10/30
CPCA61K9/19A61K47/42A61K47/36A23L33/125A23L33/185A23P10/30A23V2002/00A23V2200/30A23V2200/224A23V2250/51A23V2250/548A23V2250/5482A23V2300/44A23V2300/14
Inventor 陈丽春
Owner ZHEJIANG GONGSHANG UNIVERSITY
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