Medical use of hyperin in relieving of human aortic endothelial cell injury

A technology of hyperin and endothelial cells, applied in the field of medicine, can solve problems such as the undiscovered protective effect of hyperin, and achieve the effect of reducing endothelial damage

Inactive Publication Date: 2020-05-15
LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no technical report on the protective effect of hyperin on human aortic endothelial cell injury

Method used

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  • Medical use of hyperin in relieving of human aortic endothelial cell injury
  • Medical use of hyperin in relieving of human aortic endothelial cell injury
  • Medical use of hyperin in relieving of human aortic endothelial cell injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Experimental study on the protective effect of hyperoside on the injury of human aortic endothelial cells induced by oxidized low-density lipoprotein (ox-LDL).

[0023] 1. Experimental materials.

[0024] 1.1 Experimental cells.

[0025] Human aortic endothelial cells (HAECs) were purchased from Shanghai Fuheng Biotechnology Co., Ltd.

[0026] 1.2 Drugs and reagents.

[0027] Hyperin (purity ≥ 98%, Shanghai Yuanye Biotechnology); human oxidized low-density lipoprotein (Beijing Synbiotics); ECM medium (Scien Cell, USA); 0.25% trypsin (gibco, USA); fetal bovine Serum (gibco, USA); dimethyl sulfoxide (Shanghai Yuanye Biotechnology); PBS (Tianjin Haoyang Huake Biotechnology); CCK-8 kit (Suzhou Yuheng Biology); human NO, eNOS, iNOS, MDA, IL- 6 ELISA kit (Shanghai Enzyme Biotechnology); SDS-PAGE gel preparation kit, BCA protein concentration determination kit, 5×Tris-glycine electrophoresis buffer, 10× electrotransfer solution, 10×TBST, 10× Ponceau staining solu...

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Abstract

The invention belongs to the technical field of medicines, and particularly relates to medical use of hyperin in relieving of human aortic endothelial cell injury. The invention discloses applicationof the hyperin in preparation of drugs for preventing and treating the human aortic endothelial cell injury through independent use or combined use with other drugs. Experiments prove that the hyperinin each dose group is capable of relieving the injury of ox-LDL-induced HAECs and has concentration dependence, so that the hyperin is capable of protecting the injury of the ox-LDL-induced HAECs invitro, and the effects of the hyperin are optimal at two concentrations of 1umol / L and 10umol / L. The application range of the hyperin is broadened; by researching application of the hyperin in the protection drugs for the human aortic endothelial cell injury induced by oxidized low-density lipoprotein and further exploring biological activity of the hyperin, fundamental foundations and experimental evidences are provided for the prevention and treatment of human aortic endothelial cell injury diseases; and the hyperin is wide in source, safe in use and low in cost and has relatively wide application prospects.

Description

technical field [0001] The invention belongs to the technical field of medicine and relates to the application of hyperoside, in particular to the medical application of human aortic endothelial cell injury. Background technique [0002] Human aortic endothelial cells (HAECs) injury plays an important role in the pathological process of cardiovascular diseases. It is an important tool for elucidating the mechanism of endothelial injury and screening drugs for endothelial cell protection. It is also a new trend in the field of human cardiovascular disease treatment. Human aortic endothelial cells serve as a barrier between human blood and the arterial wall. They have physiological functions such as reducing vascular permeability, inhibiting cell migration and chemotaxis, and inhibiting platelet aggregation and adhesion. At the same time, they can synthesize and release vasodilators (such as nitric oxide) and vasoconstrictors (such as endothelin-1) to control the balance of ao...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P9/10A61P9/04A61P9/12
CPCA61K31/7048A61P9/10A61P9/04A61P9/12
Inventor 胡丽萍刘驰
Owner LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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