Medical use of hyperin in relieving of human aortic endothelial cell injury
A technology of hyperin and endothelial cells, applied in the field of medicine, can solve problems such as the undiscovered protective effect of hyperin, and achieve the effect of reducing endothelial damage
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[0022] Example 1 Experimental study on the protective effect of hyperoside on the injury of human aortic endothelial cells induced by oxidized low-density lipoprotein (ox-LDL).
[0023] 1. Experimental materials.
[0024] 1.1 Experimental cells.
[0025] Human aortic endothelial cells (HAECs) were purchased from Shanghai Fuheng Biotechnology Co., Ltd.
[0026] 1.2 Drugs and reagents.
[0027] Hyperin (purity ≥ 98%, Shanghai Yuanye Biotechnology); human oxidized low-density lipoprotein (Beijing Synbiotics); ECM medium (Scien Cell, USA); 0.25% trypsin (gibco, USA); fetal bovine Serum (gibco, USA); dimethyl sulfoxide (Shanghai Yuanye Biotechnology); PBS (Tianjin Haoyang Huake Biotechnology); CCK-8 kit (Suzhou Yuheng Biology); human NO, eNOS, iNOS, MDA, IL- 6 ELISA kit (Shanghai Enzyme Biotechnology); SDS-PAGE gel preparation kit, BCA protein concentration determination kit, 5×Tris-glycine electrophoresis buffer, 10× electrotransfer solution, 10×TBST, 10× Ponceau staining solu...
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