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Sheep GHR gene insertion/deletion polymorphism detection primer pair, kit containing primer pair, method by using primer pair and application of method

A technology for polymorphism detection and detection methods, applied in biochemical equipment and methods, microbial measurement/testing, DNA/RNA fragments, etc., can solve problems such as undiscovered patent documents, and achieve improved breeding speed, low cost effect

Active Publication Date: 2020-05-15
天津奥群牧业有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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[0007] Through searching, no published patent documents related to this patent application have been found

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  • Sheep GHR gene insertion/deletion polymorphism detection primer pair, kit containing primer pair, method by using primer pair and application of method
  • Sheep GHR gene insertion/deletion polymorphism detection primer pair, kit containing primer pair, method by using primer pair and application of method
  • Sheep GHR gene insertion/deletion polymorphism detection primer pair, kit containing primer pair, method by using primer pair and application of method

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Embodiment Construction

[0030] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are illustrative, not restrictive, and cannot limit the protection scope of the present invention.

[0031] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0032] A primer pair for detecting insertion / deletion polymorphism of sheep GHR gene, said primer pair can be used for PCR amplification of 23-bp insertion / deletion polymorphism site of sheep GHR gene NC_019473.1:g.31984400_31984422.

[0033] Preferably, the primer pair is:

[0034]Upstream primer: SEQ NO.1 5'-GCTTCTTGCCCAACCCAATG-3'(20nt);

[0035] Downstream primer: SEQ NO.2 5'-CTGGGCAGTGGAGGAGAAAG-3' (20nt).

[0036] Application of the above-mentioned primer pair for detection of insertion / deletion polymorphism o...

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Abstract

The present invention relates to a sheep GHR gene insertion / deletion polymorphism detection primer pair. The primer pair can be used for PCR amplification of sheep GHR gene NC_019473.1:g. 31984400_31984422 site 23-Bp insertion / deletion polymorphism site. The primer pair can detect genotypes of the insertion / deletion polymorphism site simply, quickly, at low cost and accurately, and the detected insertion / deletion polymorphism site can be used as a molecular marker site for the number of lambs produced by sheep, thereby accelerating establishment of sheep populations with excellent lamb numbertraits and increasing speed of breeding of improved varieties.

Description

technical field [0001] The invention belongs to the technical field of biotechnology and domestic animal breeding, in particular to a detection primer pair, kit, method and application of sheep GHR gene insertion / deletion polymorphism. Background technique [0002] Sheep are deeply loved by consumers for their meat, milk and cashmere production properties. With the improvement of people's living standards, the society's demand for mutton, goat milk, cashmere and other products is getting higher and higher. In order to achieve the goal of high-yield, high-quality and efficient sheep breeding, people have studied sheep at the molecular level, using DNA polymorphisms as markers to conduct genetic analysis and research on the gene structure and function of sheep, and through screening and sheep production. DNA marker loci closely related to litter size traits are detected, and according to the association between their gene polymorphisms and litter size traits, the establishment...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2531/113C12Q2565/125Y02P60/87
Inventor 张清峰蓝康澍潘传英曹如林春建郝坤杰韩旭飞兰天鑫
Owner 天津奥群牧业有限公司