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A purine base plant immune inducer and its application

An immune inducer and plant technology, applied in plant growth regulators, plant growth regulators, applications, etc., can solve the problems of difficult prevention and control of plant diseases, lack of effective fungicides for oomycetes, etc.

Active Publication Date: 2021-05-04
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to control the diseases caused by this group of pathogenic organisms, mycologists and plant pathologists from all over the world have conducted a lot of research on Phytophthora from different angles, but due to the difficulty in obtaining long-lasting resistance sources from host plants and the lack of Effective fungicide against oomycetes, therefore, difficult to control plant diseases caused by oomycetes

Method used

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  • A purine base plant immune inducer and its application
  • A purine base plant immune inducer and its application
  • A purine base plant immune inducer and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1. Experimental crop: rice (japonica rice Zhonghua 11).

[0043] 2. Planting method: Rice is planted in a greenhouse with 16 hours of light and 8 hours of darkness alternately every day. The cultivation temperature is 26°C at room temperature and the air humidity is 60-70%. The greenhouse is located in the State Key Laboratory of Crop Biology, Shandong Agricultural University .

[0044] 3. Test method:

[0045] The plant immune inducer (containing guanine solution of 100ng / ml and 0.05% Silwet L-77) was sprayed on the paddy rice leaves and stems of 8 weeks of cultivation, and contained only 0.05% Silwet L-77 aqueous solution as a control. Two hours later, the cultured sheath blight bacteria wrapped the wooden matchstick, embedded the fungus-carrying wooden matchstick into the rice leaf sheath by the embedding method, and sealed it with a plastic wrap to keep it moist. After 4 days of inoculation, the diseased part of the rice was cut off and the phenotype of the disea...

Embodiment 2

[0050] 1. Experimental crop: rice (japonica rice Zhonghua 11).

[0051] 2. Planting method: Rice is planted in a greenhouse with 16 hours of light and 8 hours of darkness alternately every day. The cultivation temperature is 26°C at room temperature and the air humidity is 60-70%. The greenhouse is located in the State Key Laboratory of Crop Biology, Shandong Agricultural University .

[0052] 3. Experimental method:

[0053] When the paddy rice is cultivated to the 6th week, the plant immune inducer (guanine solution containing 100ng / ml and 0.05% Silwet L-77) is sprayed on the paddy rice blade, and with only containing 0.05% Silwet L-77 aqueous solution as a comparison. After 2 hours, with 10mM MgCl 2 The bacterial streak RS105 cultured on the plate was eluted, and the OD value was adjusted to 0.005, and the rice leaves were injected and inoculated with a sterilized medical syringe. After 10 days of inoculation, the diseased leaves were sampled and photographed, and the di...

Embodiment 3

[0057] 1. Experimental crop: rice (japonica rice Zhonghua 11).

[0058] 2. Planting method: Rice is planted in a greenhouse with 16 hours of light and 8 hours of darkness alternately every day. The cultivation temperature is 26°C at room temperature and the air humidity is 60-70%. The greenhouse is located in the State Key Laboratory of Crop Biology, Shandong Agricultural University .

[0059] 3. Test method:

[0060] Rice was sprayed with 0.05% Silwet L-77 aqueous solution (control) and 100ng / ml guanine (containing 0.05% Silwet L-77) after 2 hours, with 10mM MgCl 2 The bacteria strain PXO99A cultured on the plate was eluted, and the OD value of the solution was adjusted to 0.5, and the scissors with a frosted surface were dipped in the bacterial solution, and the rice leaf tip was sheared and inoculated. After 18 days of inoculation, the The diseased leaves were sampled and photographed, and the length of the lesion was measured.

[0061] 4. Test results:

[0062] image...

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Abstract

The invention discloses a purine base plant immune inducer and application thereof, belonging to the technical field of biological pesticides. The present invention finds that guanine can improve the defense response of plants and reduce the incidence of disease, especially has a better control effect on crop oomycete diseases. Therefore, guanine and its structural analogues can be used as active ingredients of plant immune inducers . The plant immune inducer prepared by using guanine or its structural analogues as active ingredients in the present invention mainly adopts the method of spraying in application, which is simple and convenient to operate, and can be sprayed in advance during the period of high incidence of plant diseases, which can effectively prevent diseases Occurs on a large scale.

Description

technical field [0001] The invention relates to the technical field of biological pesticides, in particular to a purine base plant immune inducer and its application. Background technique [0002] my country is a large agricultural country, and food is a necessity for the people, which is of great significance to the stable growth of the national economy. But current crops are heavily diseased and billions of dollars worth of crops are lost every year due to different diseases caused by pathogens. For a long time, the main method for farmers to prevent and control diseases is chemical control. By spraying pesticides, the pathogenic bacteria are directly targeted to kill the pathogenic bacteria quickly, directly and comprehensively, so as to achieve the purpose of disease control. However, due to problems such as high dosage of pesticides and unscientific application of pesticides, it is easy to cause environmental pollution, crop damage, pathogenic bacteria resistance and o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/90A01N25/30A01P21/00A01P1/00A01P3/00
CPCA01N25/30A01N43/90
Inventor 丁新华路冲冲李洋储昭辉王露露
Owner SHANDONG AGRICULTURAL UNIVERSITY
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