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Application of sclareol and sclareolide in resisting filovirus infection

A technology of sclareolide and filovirus, which is applied in the field of medicine and can solve the problems of no antiviral activity

Active Publication Date: 2020-06-12
INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Although it has been reported that sclareol and sclareolide have various antifungal and antitumor effects, there is no report on their antiviral activity (including filoviruses and other types of viruses) after literature search

Method used

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  • Application of sclareol and sclareolide in resisting filovirus infection
  • Application of sclareol and sclareolide in resisting filovirus infection
  • Application of sclareol and sclareolide in resisting filovirus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1. Principle of Screening Model

[0047] Filovirus entry into host cells is the first step in virus infection, and inhibiting virus entry can effectively block virus infection. The glycoprotein (Glycoprotein, GP) on the surface of the filovirus envelope is a key protein in the process of filovirus entry.

[0048] We synthesized the envelope GP gene of Zaire type Ebola virus (EBOV-GP, Gene Accession No.L11365), the envelope GP gene of Sudan type Ebola virus (SUDV-GP, Gene Accession No.FJ968794.1) , Tai Forest type Ebola virus envelope GP gene (TAFV-GP, Gene Accession No.FJ217162.1), Marburg virus envelope GP gene (Marburg-GP, Gene Accession No.NC_001608.3) and Cueva virus Envelope GP gene (Cueva-GP, Gene Accession No. JF828358). Expression of GP protein plasmid and pNL4-3-Luc-R by co-transfection - E. - , Filovirus GP can be obtained as a filovirus recombinant virus HIV / Filovirus-GP that coats the HIV core[Chen Q,Tang K,Zhang X,Chen P,Guo Y.Establishment of p...

Embodiment 2

[0049] Example 2. Inhibition of Sclareol and Sclareolide to Filamentous Recombinant Virus Infection

[0050] In the present invention, Zaire type Ebola virus (EBOV, Gene Accession No.L11365), Sudan type Ebola virus (SUDV, Gene Accession No.FJ968794.1), Tai Forest type Ebola virus ( Anti-filovirus infection of TAFV, Gene Accession No.FJ217162.1), Marburg virus (Gene Accession No.NC_001608.3) and Cueva virus (Gene Accession No.JF828358) to sclareol and sclareolide Action for pharmacological activity evaluation:

[0051] Recombinant virus preparation [Chen Q, Tang K, Zhang X, Chen P, Guo Y. Establishment of pseudovirus infection mouse models for in vivo pharmacodynamics evaluation offilovirus entry inhibitors. Acta Pharm Sin B. 2018; 8(2): 200-208.] : Co-transfected with 2 μg pcDNA3.1 / EBOV-GP plasmid (or 2 μg pcDNA3.1 / SUDV-GP plasmid or 2 μg pcDNA3.1 / TAFV-GP plasmid or 2 μg pcDNA3.1 / Marburg-GP plasmid or 2 μg pcDNA3.1 / Cueva -GP plasmid) and 2 μg pNL4-3-Luc-R - E. - Put the pl...

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PUM

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Abstract

The invention discloses application of sclareol and sclareolide in resisting filovirus infection, wherein the application comprises application of sclareol or / and sclareolide in prevention or treatment of filovirus infection or combined application of sclareol or / and sclareolide and other antiviral drugs.

Description

technical field [0001] The invention relates to the application of sclareol and sclareolide in resisting filovirus infection, and belongs to the technical field of medicine. The present invention includes the single or combined application of sclareol and sclareolide in preventing or treating filovirus infection. Background technique [0002] Filoviruses belong to the Filoviridae family, also known as Filoviruses, which are divided into three genera, namely Ebolavirus, Marburgvirus and Cuevavirus. Viruses (Cuevavirus). Ebola virus disease (EVD), caused by Ebola virus, is a major and severe infectious disease that seriously endangers public safety and human health, with a fatality rate as high as 25%-90% [Sanchez, A.; Geisbert, TW .; Feldmann, H. Filoviridae: Marburg and Ebola viruses. In: Knipe, DM.; Howley, PM., editors. Fields virology. Philadelphia: Lippincott Williams & Wilkins; 2006.1409-1448.]. Marburg virus is also fatal, with a fatality rate of 50-80% [Nonfatal ca...

Claims

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Application Information

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IPC IPC(8): A61K31/047A61K31/365A61P31/12A61P31/14
CPCA61K31/047A61K31/365A61P31/12A61P31/14Y02A50/30
Inventor 郭颖陈勍唐克
Owner INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
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