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Method for simultaneously and rapidly detecting amino sugar, neutral sugar and uronic acid in seaweed

A technology of amino sugar and uronic acid, applied in the field of analysis and detection of sugar compounds in the food industry, can solve problems such as reducing column efficiency, polluting chromatographic column, affecting separation effect, etc., and achieves improved applicability, accurate detection results, and improved The effect of extraction efficiency

Active Publication Date: 2020-06-12
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Seaweed contains a lot of protein, and the presence of protein contaminates the chromatographic column, which affects the separation effect and reduces column efficiency. The sample pretreatment in this document did not remove protein impurities; in addition, the types of sugars detected in this document are also less; Therefore, there is an urgent need for a sugar detection method that simultaneously detects multiple sugars, does not require derivatization, and has accurate detection results and high stability.

Method used

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  • Method for simultaneously and rapidly detecting amino sugar, neutral sugar and uronic acid in seaweed
  • Method for simultaneously and rapidly detecting amino sugar, neutral sugar and uronic acid in seaweed
  • Method for simultaneously and rapidly detecting amino sugar, neutral sugar and uronic acid in seaweed

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Effect test

Embodiment 1

[0049] The method for rapid detection of amino sugar, neutral sugar and uronic acid in seaweed mainly comprises the following steps:

[0050] (1) Extraction of kelp polysaccharides from kelp: firstly wash the freshly picked kelp to remove impurities such as silt and salt stains on the surface, dry it in an oven at 50°C, crush it into kelp powder with a crusher, and pass it through a 60-mesh mesh sieve , stored at -20°C for later use. Take 5g of kelp powder and remove impurities such as pigment and grease with 10 times the volume of absolute ethanol, and dry it in a 50°C drying oven to constant weight. Add 30 times of ultrapure water, use an ultrasonic cell pulverizer to treat for 30 minutes, place in a constant temperature water bath at 60°C for 3 hours, and centrifuge to get the supernatant. Concentrate on a rotary evaporator at 60°C to 1 / 4 of the original volume, add absolute ethanol to a mass fraction of 80%, and overnight at 4°C. Centrifuge, take the precipitate, redisso...

Embodiment 2

[0072] Influence of eluting phase and elution system on detection results

[0073] (1) Effect of different sodium hydroxide concentrations on sugar chromatograms

[0074] Nine sugar compounds (Fuc, Glc, GalN, GlcN, Gal, Man, Fruc, Rib, and Lac) were eluted isocratically with 5, 10, 20, 25, and 30mmol / L NaOH solutions respectively, and different hydrogen oxidation levels were detected. The effect of sodium concentration on the resolution and peak shape of chromatographic peaks, the results are as follows figure 1 shown. During the test, it was found that with the increase of NaOH concentration, the retention time of each chromatographic peak shortened and the response value increased, which may be due to the increase of monosaccharide dissociation degree with the increase of NaOH concentration. This is due to the OH separated by the concentration of the eluent NaOH solution - It can affect the separation of monosaccharides from two directions. On the one hand, with the incre...

Embodiment 3

[0080] (1) linear relationship and detection limit of detection method of the present invention

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Abstract

The invention discloses a method for simultaneously and rapidly detecting amino sugar, neutral sugar and uronic acid in seaweed, and belongs to the technical field of analysis and detection of carbohydrate compounds in the food industry. The method comprises the following steps: extracting a seaweed polysaccharide crude product from a to-be-detected sample by adopting an ultrasonic-assisted waterextraction and alcohol precipitation method; removing protein in the extracted algal polysaccharide crude product by using a Sevage method, and hydrolyzing algal polysaccharide by using trifluoroacetic acid; after hydrolysis is completed, adding NaOH to neutralize the acidity of hydrolysate, and using the hydrolysate passes through a 0.22 micron filter membrane and then is used for detection of anion chromatography-pulse ampere detector. The method provided by the invention is simple and rapid, does not need derivation, greatly improves the applicability and recovery rate of the method, solves the technical problem of low efficiency when multiple detection technologies, multiple instruments and multiple pretreatment methods are adopted to determine multiple components at present, greatlyimproves the detection efficiency, and reduces the detection cost.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection of sugar compounds in the food industry, and in particular relates to a method for simultaneous rapid detection of amino sugar, neutral sugar and uronic acid in seaweed. Background technique [0002] The world today has entered a resource and environment bottleneck period, and the development of marine resources is a strategic choice for sustainable development. Marine life has unique advantages over terrestrial life. my country is rich in marine resources, and seaweed processing and its high-value utilization have become an important field of comprehensive utilization of marine resources in my country. Seaweed polysaccharides have significant effects in reducing blood fat, anti-oxidation, anti-coagulation, anti-tumor, anti-fatigue, anti-inflammation, immune regulation and neuroprotection, and are more and more widely used in the fields of biochemistry, health food and clinical med...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/8679
Inventor 王联珠尹大芳孙晓杰郭莹莹江艳华彭吉星刘芬
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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