Magnetic stirrer for preparing monoclonal antibody

Abstract

The invention discloses a magnetic stirrer for preparing a monoclonal antibody. The magnetic stirrer comprises a bottom plate; two oppositely-arranged supporting rods are fixed to the upper surface ofthe bottom plate; a lifting rod is arranged in each supporting rod; a lifting screw rod is arranged in each lifting rod; a damping cross rod is fixed at the top end of each lifting rod; a motor fixing frame is arranged between the two oppositely-arranged damping cross rods; a stirring motor is fixed on the motor fixing frame; a stirring rotating shaft is arranged at the output end of the stirringmotor; stirring blades are fixed on the stirring rotating shaft; a fixed base is also fixed on the upper surface of the bottom plate; a fixed barrel is arranged on the fixed base; a stirring barrel is arranged in the fixed barrel, the damping cross rods, damping connecting blocks and the fixed base are sued for counteracting generated vibration, the service life of the equipment is prolonged, thewashing efficiency of the cover plate is guaranteed, then the preparation efficiency of monoclonal antibodies and threaded connection of a fixed barrel and the stirring barrel are guaranteed, the stirring barrel can be taken down from the fixed barrel, and cleaning of the stirring barrel is facilitated.

Description

technical field [0001] The invention belongs to the field of monoclonal antibody preparation, and in particular relates to a magnetic stirrer for monoclonal antibody preparation. Background technique [0002] Monoclonal antibody is a highly uniform antibody produced by a single B cell clone that only targets a specific epitope. It is usually prepared by hybridoma technology. Hybridoma antibody technology is based on cell fusion technology and will have secretion specificity. The sensitized B cells with the ability of anti-antibodies and the myeloma cells with unlimited reproduction ability are fused into B cell hybridomas, so the cultivation of hybridoma cells is very important in the process of preparing monoclonal antibodies; [0003] After the preparation of the hybridoma cells is completed, the antibody needs to be detected. The antibody can be detected by immunofluorescence technology. The immunofluorescence technology detects the antibody. The traditional magnetic sti...

AI Technical Summary

Problems solved by technology

[0003] After the preparation of the hybridoma cells is completed, the antibody needs to be detected. The antibody can be detected by immunofluorescence technology. The immunofluorescence technology detects the antibody. The traditional magnetic stirrer is mostly non-sealed. Washing the cover plate with the fixed bacterial suspension will cause the cover plate to be polluted by the outside and affect the washing effect of the cover plate, resulting in non-specific antibody binding, increasing False positive, and a vortex flow will be generated during the stirring process. The violently swirling liquid often causes reciprocating impulse waves. The reciprocating impulse waves combined with the effect of the vortex will have an impact on the stirring shaft and cause the entire equipment to vibrate. Long-term The shock of time greatly shortens the service life of the equipment, which in turn affects the preparation of monoclonal antibodies

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