Preparation method of conductive hydrogel and its cell impedance sensing detection method
A conductive hydrogel and detection method technology, applied in the direction of measuring resistance/reactance/impedance, material impedance, measuring device, etc., can solve the problems of inefficient and stable charge transfer, affecting cell impedance sensing and detection, etc., and achieve efficient and stable charge transfer effect
Active Publication Date: 2021-08-17
PEKING UNIV
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Problems solved by technology
[0004] In order to solve the technical problem in the technical field of cell impedance sensing detection that the charge transfer in the detection process cannot be efficiently and stably caused by metal electrodes due to detection limitations, which further affects the technical problem of cell impedance sensing detection, the invention discloses a preparation of a conductive hydrogel Method and Cell Impedance Sensing Detection Method thereof
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[0026] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be described in further detail below in conjunction with specific embodiments and with reference to the accompanying drawings.
[0027] As a three-dimensional (3D) material, hydrogel is capable of absorbing large amounts of water while maintaining its dimensional stability, maintaining the 3D structural integrity of the hydrogel in the swollen state through physical or chemical crosslinking. Chemically crosslinked networks have permanent knots, while physically crosslinked networks have transient knots caused by polymer chain entanglements or physical interactions such as ionic interactions, hydrogen bonds, or hydrophobic interactions. Hydrogels containing "sensor" properties can undergo reversible volume phase transitions or gel-sol phase transitions upon small changes in environmental conditions. These stimuli-responsive types of hydrogels are al...
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Abstract
The invention discloses a method for preparing a conductive hydrogel and a cell impedance sensing detection method thereof. The method for preparing the conductive hydrogel includes: forming a conductive chip with a micro-electrode array; forming a cell culture chamber through the conductive chip; forming a polymerized hydrogel on the microelectrode array of the cell culture chamber; and forming a conductive hydrogel with a micropattern by polymerizing the hydrogel. The present invention prepares a soft conductive hydrogel with a micropattern of an integrated interpenetrating network structure (IPN structure) on a conductive chip of a microelectrode array by an electrochemical deposition method, and the conductive hydrogel can effectively replace cell impedance sensing The metal electrodes in the technical field, combined with the impedance meter online detection system, effectively realize the efficient and stable charge transfer in the process of cell impedance sensing and detection, making the cell impedance detection achieve the technical effect of label-free, full-range dynamic and real-time analysis.
Description
technical field [0001] The invention belongs to the technical field of cell impedance sensing detection, and in particular relates to a preparation method of a micropattern conductive hydrogel and a cell impedance sensing detection method thereof. Background technique [0002] Electric Cell Impedance Sensing (ECIS) is a cell analysis method based on AC impedance technology. Its principle is to evaluate the physiological and pathological state of cells by measuring the impedance value change between cells and substrate electrodes. It can real-time Monitor dynamic biological processes such as cell growth, proliferation, toxicity, adhesion, and morphological changes. As a label-free, dynamic and real-time analysis method, ECIS has been widely used in cell research. In this field, AC impedance methods for cell analysis can be roughly divided into two categories: non-Faradic Electric Impedance Sensing (NFEIS) and Faraday Impedance Sensing (Faradic Electric Impedance Sensing, FEI...
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IPC IPC(8): C08J3/075C08L51/02C08L65/00C08F251/00C08F220/56G01N27/02G01R27/02
CPCC08F251/00C08J3/075C08J2351/02C08J2365/00C08J2451/02C08J2465/00G01N27/02G01R27/02C08F220/56
Inventor 黄建永姜楠
Owner PEKING UNIV