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Measurement method combining scattering turbidimetry and transmission turbidimetry

A technology of turbidimetry and measurement method, which is applied in the direction of transmittance measurement, scattering characteristic measurement, etc., which can solve the problems of many clots and poor quantitative measurement results, and achieve the effect of high measurement accuracy

Pending Publication Date: 2020-06-23
PUREBIO LAB NINGBO
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Problems solved by technology

[0004] As well-known immunoturbidimetric measurement techniques, turbidimetry and turbidimetry have the following advantages and disadvantages: Generally speaking, turbidimetry can detect samples with high sensitivity for low-concentration samples, but it is not effective for samples with high concentrations. , more agglutinates are generated, and the quantitative measurement results become worse due to the influence of multiple scattering
Therefore, except that the measured quantities in the low-concentration interval and the high-concentration interval are the only output options, there is a problem of selecting the two measured quantities in the overlapping intervals of the two detection methods

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  • Measurement method combining scattering turbidimetry and transmission turbidimetry
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  • Measurement method combining scattering turbidimetry and transmission turbidimetry

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Embodiment Construction

[0024] The present invention is further described below through specific embodiments, but the present invention is not limited to the following specific embodiments.

[0025] A combined nephelometric and nephelometric measurement method, wherein:

[0026] The turbidimetric method is based on the following principle. After parallel light passes through suspensions and colloidal solutions with tiny particles, the intensity of incident light is weakened due to the influence of light absorption. According to the Lambert-Beer law, this phenomenon can be used Formula (1) expresses:

[0027] E=KC Formula (1)

[0028] In the formula, E: change rate of absorbance; K: constant; C: concentration of solution.

[0029] The nephelometric method is based on the following principle. When the particle is irradiated by light and the particle diameter is greater than half of the wavelength of the incident light wave, light scattering will occur. According to the Rayleigh scattering law, this p...

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Abstract

The invention relates to the technical field of sample concentration measurement, in particular to a measurement method combining a scattering turbidimetry and a transmission turbidimetry. The randomerror epsilon and the offset f of each turbidimetry are combined, the total error TE of the two turbidimetry is obtained respectively, then the two total errors are compared, if the two total errors are different, the measurement method with the small total error is selected as a quasi value, and if the two total errors are the same, the mean value of the measurement values of the scattering turbidimetry and the scattering turbidimetry is taken as the quasi value. The measurement method is high in measurement accuracy.

Description

technical field [0001] The invention relates to the technical field of sample concentration measurement, in particular to a measurement method combining nephelometric and nephelometry. Background technique [0002] The purpose of a clinical test is to provide an accurate measurement of the test sample for its intended use. This measurement can be used in clinical diagnosis, monitoring and screening of diseases. The measurement process is usually a process in which a specific analysis device converts the response signal into a quantity based on the response signal generated after the measured substance reacts with a specific reagent. The analytes of human-derived detection samples have different molecular order sizes, and there are various analytical methods with different detection sensitivities applied to measure substances of different molecular orders. [0003] Chemiluminescent immunoassays typically detect pg, ng levels of trace measurement samples. General constant d...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/49G01N21/59
CPCG01N21/49G01N21/59
Inventor 卓伟奇王剑王远周敏霞楼洁燕
Owner PUREBIO LAB NINGBO
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