Unlock instant, AI-driven research and patent intelligence for your innovation.

Raman spectrum detection method for rapidly determining content of traditional Chinese medicine polysaccharide

A technology for Raman spectrum detection and polysaccharide content, which is applied in the directions of Raman scattering and material excitation analysis, can solve the problems that there are no reports on polysaccharide Raman spectrum analysis methods, and achieve improved detection efficiency and product quality, short measurement time, The effect of easy operation

Inactive Publication Date: 2020-06-23
ZHEJIANG UNIV
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are Raman spectroscopic methods for the determination of monosaccharides and disaccharides in honey, beverages and other products in the literature, but there is no Raman spectroscopic method for the determination of polysaccharides.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Raman spectrum detection method for rapidly determining content of traditional Chinese medicine polysaccharide
  • Raman spectrum detection method for rapidly determining content of traditional Chinese medicine polysaccharide
  • Raman spectrum detection method for rapidly determining content of traditional Chinese medicine polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] What this embodiment provides is the content determination of the polysaccharide in Codonopsis pilosula extract, specifically according to the following steps:

[0054] (1) Preparation of Codonopsis pilosula extract sample

[0055] Accurately weigh 1 g of Codonopsis polysaccharide (containing 70% polysaccharide, determined by phenol-sulfuric acid method) extract and dissolve it ultrasonically in 50 mL of water, as Codonopsis polysaccharide stock solution. Serially dilute stock solution with water to obtain 0.070mg / mL-7.350mg / mL Codonopsis polysaccharide solution (each concentration interval 0.070mg / mL), 7.420mg / mL-9.380mg / mL Codonopsis polysaccharide solution (each concentration interval 0.140mg / mL) a total of 120 Codonopsis polysaccharide solutions were obtained, and these solutions were used as calibration set samples. In addition, Codonopsis polysaccharide solutions of 1.540mg / mL, 3.080mg / mL, 4.620mg / mL, 6.160mg / mL and 7.700mg / mL were prepared, and each solution wa...

Embodiment 2

[0067] What this embodiment provides is the determination of the content of polysaccharides in Polygonatum extract, specifically according to the following steps:

[0068] (1) Preparation of Polygonatum Extract Sample

[0069] Accurately weigh 1g Polygonatum polysaccharide extract (containing 70% polysaccharide, determined by phenol-sulfuric acid method) and ultrasonically dissolve it in 50mL water, as Polygonatum polysaccharide stock solution. Serially dilute the stock solution with water to obtain 0.070mg / mL-7.350mg / mL Polygonatum polysaccharide solution (each concentration interval 0.070mg / mL), 7.420mg / mL-9.380mg / mL Polygonatum polysaccharide solution (each concentration interval 0.140mg / mL) to obtain 120 Polygonatum polysaccharide solutions in total, and these solutions are used as calibration set samples. In addition, Polygonatum polysaccharide solutions of 1.400mg / mL, 2.800mg / mL, 4.200mg / mL and 7.000mg / mL were prepared, and each solution was parallelized in 6 copies, a...

Embodiment 3

[0081] What this embodiment provides is the polysaccharide content determination of the sample polysaccharide in the Codonopsis-Polygonatum extract process, specifically according to the following steps:

[0082] (1) Collection of Codonopsis pilosula-Polygonatum extraction process samples

[0083] Add 0.75kg of Codonopsis Codonopsis and 1.0kg of Polygonatum into a 30L extraction tank, extract with 14L of water at 100°C for 120min, collect the extract, and then use 10.5L of water for a second extraction for 90min. During the first extraction, samples were collected every 5 minutes between 0-60 minutes, and samples were collected every 10 minutes between 60 minutes and 120 minutes. During the second extraction, samples were collected every 5 minutes during 0-45 minutes, and samples were collected every 10 minutes during 45-90 minutes. 33 samples were collected for each batch of medicinal materials, and there were 5 batches of medicinal materials in total. Among them, a total o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of traditional Chinese medicine detection, and particularly relates to a Raman spectrum detection method for rapidly determining the polysaccharide content in traditional Chinese medicine codonopsis pilosula and polygonatum kingianum and the polysaccharide content in the pharmaceutical process thereof. The method specifically comprises the following steps: 1) respectively preparing traditional Chinese medicine samples with different polysaccharide contents as calibration sets; 2) collecting Raman spectra of the calibration set and removing abnormal samples inthe Raman spectra; 3) selecting a modeling waveband by using a CARS algorithm and establishing a PLS model; and 4) collecting the Raman spectrum of a sample to be detected, and fitting the obtained spectrum to the model to obtain the polysaccharide content in the sample to be detected. The method disclosed by the invention does not need any sample pretreatment, is simple and convenient to operate, green and environment-friendly, not only greatly shortens the detection time of the polysaccharide content in the traditional Chinese medicine, but also realizes real-time monitoring of the polysaccharide content in the medicine production process, and greatly improves the detection efficiency and the product quality.

Description

technical field [0001] The invention belongs to the field of detection of traditional Chinese medicines, and in particular relates to a Raman spectrum detection method for rapidly determining the content of polysaccharides in Chinese medicines Codonopsis pilosula and Rhizoma Polygonatum and their pharmaceutical preparations. Background technique [0002] Polysaccharides are the main components of plants, and many polysaccharides have important pharmacological activities, so polysaccharides are used as quality indicators of many traditional Chinese medicines. Therefore, there is an urgent need for a method for monitoring polysaccharide content in the process of traditional Chinese medicine pharmacy. [0003] Codonopsis pilosula and sealwort are two important traditional Chinese medicines. Among them, Codonopsis pilosula is a traditional nourishing medicine, which strengthens the spleen and lungs, nourishes blood and promotes body fluids; sealwort is a traditional precious nou...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/65
Inventor 程翼宇王书芳
Owner ZHEJIANG UNIV