Composition and method for preserving nucleic acid in urine

Abstract

The invention provides a composition and method for preserving nucleic acid in urine. The composition provided by the invention contains the following components in percentage by weight: 2%-20% of a preservative, 1%-10% of a quenching agent, 2%-40% of a nuclease inhibitor, 5%-60% of a hydrophilic polymer, 1%-30% of a penetrating agent and 0.01%-10% of a metabolic inhibitor. The composition is capable of improving the stability of free nucleic acids in a biological sample and preventing release of nucleic acids in a karyocyte genome, and the separated free nucleic acids are widely suitable formass clinical diagnosis, development and application.

Description

technical field [0001] The invention relates to the field of biological samples, in particular to a composition and method for preserving nucleic acids in urine. Background technique [0002] Liquid biopsy, the method of obtaining biomarkers directly from bodily fluids (such as blood or urine) rather than solid tissue, is a simple, non-invasive alternative to surgical biopsies. Cell-free nucleic acids in blood circulation can provide key information about diseases that are difficult to detect with conventional standard methods. [0003] However, compared with peripheral blood, urine has higher nuclease content, more metabolites, and greater pH range fluctuations, so the biological environment of free nucleic acids in urine is more complex than that in blood. If there are white blood cells in the urine or if the epithelial cells break down and the genomic nucleic acid degrades into the urine, or if the nuclease in the urine is not inhibited, the initial nucleic acid will be ...

AI Technical Summary

Problems solved by technology

[0003] However, compared with peripheral blood, urine has higher nuclease content, more metabolites, and greater pH range fluctuations, so the biological environment of free nucleic acids in urine is more complicated than that in blood

If there are white blood cells in the urine or if the epithelial cells break down and the genomic nucleic acid degrades into the urine, or if the nuclease in the urine is not inhibited, the initial nucleic acid will be degraded in a short time, which will change the initial The content of free nucleic acid, in more cases, reduces the proportion of rare mutations, increases the background noise of the detection, and brings false negative results to subsequent detection

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