Method for detecting trace estrogen, nonylphenol and bisphenol A in complex environment matrix

Abstract

The invention discloses a method for detecting trace estrogen, nonylphenol and bisphenol A. According to the method, sludge serves as a detected complex environment matrix, and the method comprises the steps: sequentially carrying out ultrasonic extraction, liquid-liquid distribution purification, two-step solid-phase extraction purification and sample preparation treatment on the detected complexenvironment matrix to obtain a sample to be detected, wherein in the ultrasonic extraction, an isotope purification internal standard is added, and methanol is used as an extraction solvent; and detecting trace estrogen, nonylphenol and bisphenol A in the obtained sample to be detected through high performance liquid chromatography-tandem triple quadrupole mass spectrometry to obtain the concentrations of estrogen, nonylphenol and bisphenol A. The invention further discloses a method for detecting estrogen, nonylphenol and bisphenol A in the sample to be detected. According to the method, theconcentrations of estrogen, nonylphenol and bisphenol A in the matrix sludge in the complex environment can be rapidly and accurately determined. The method is simple and convenient to operate, resistant to matrix interference, good in reproducibility and high in reliability.

Description

Technical field [0001] The invention belongs to the field of hazardous substance detection, and particularly relates to a detection method of trace estrogen, nonylphenol and bisphenol A in a complex environment matrix. Background technique [0002] In recent years, environmental estrogens have attracted widespread attention from scholars at home and abroad due to their endocrine disrupting effects. There are mainly endogenous estrogens such as estrone (Estrone, E1) and 17β-estradiol (ie 17β-Estradiol, E2), estriol (ie Estradiol, E3), etc., synthetic exogenous estrogen such as 17α-ethinyl estradiol (ie 17a-Ethinylestradiol, EE2), alkylphenols such as nonylphenol ( Namely Nonylphenol, NP), Bisphenol A (ie Bisphenol A, BPA) and so on. [0003] These pollutants will be discharged into the sewage treatment system or farmland soil along with the excrement and urine. Research and investigation have shown that the current sewage treatment process cannot effectively remove environmental es...

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Problems solved by technology

[0006] Based on the existing problems in the prior art, the purpose of the present invention is to provide a detection method for trace estrogen, nonylphenol and bisphenol A in a complex environmental matrix, which can solve the problem of using the existing detection method for complex environmental matrix sludge During the sample detection, due to the complex sludge matrix and the existence of traces of estrogen, the background interference is large and the accuracy is not high.

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