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A kind of extraction method of Sapindus saponin

A technology of sapindus saponin and extraction method, applied in the direction of steroids, organic chemistry, etc., can solve problems such as unsafe, large environmental pollution, complicated process, etc., and achieve the effects of increased yield, environmental friendliness, and light color

Active Publication Date: 2021-06-29
CHANGSHA LANGLIN BIOLOGY PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The final product of this purification method has higher purity and low cost, but it still has the following disadvantages: 1. there are many factors affected, including the pH value of the solution, temperature, etc.; 2. the process is relatively complicated, and it is difficult to realize industrial production, and Use a large amount of organic reagents, which is harmful to the environment; ③This process cannot obtain two different types of Sapindus at one time
Chinese invention patent CN104292294A discloses a method for the purification of sapindus sapinin, specifically disclosing that two different types of sapindus saponins are obtained by extraction method: extracting sapindus pericarp into absolute ethanol, centrifuging Add acetone to the supernatant and carry out sedimentation centrifugation, add ether to the supernatant after centrifugation and carry out sedimentation centrifugation to obtain two different types of Sapindus saponins. However, this method has the following disadvantages: ① But this process uses A large number of organic reagents cause great pollution to the environment and are unsafe
② Not suitable for mass industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 of the present invention is: a kind of extracting method of sapindus saponin, comprises the following steps:

[0027] (1) Take 100g of sapindus fruit peel raw material, add 5 times the volume of water to soak twice, each time for 1h, collect all the soaking liquid and soaking residue respectively, and the soaking liquid is centrifuged, and the soaking liquid after centrifugation adopts ultrafiltration equipment (the molecular weight cut off 1000) to filter, soak the clear liquid in water, add 8 times the volume of 80% ethanol to the soaked residue, and extract twice in a water bath at 90°C under reflux, each extraction for 2 hours, combine the extracts, add alkali to the extracts to adjust pH=7, and carry out vacuum Concentrate, the concentration temperature is 65°C, concentrate until there is no alcohol smell, recover ethanol, centrifuge to obtain concentrated supernatant;

[0028] (2) The concentrated supernatant and the water-soaked supernatant merge, an...

Embodiment 2

[0030] Embodiment 2 of the present invention is: a kind of extracting method of sapindus saponin, comprises the following steps:

[0031](1) Take 100g of sapindus fruit peel raw material, add 8 times the volume of water to soak 3 times, 2 hours each time, collect all the soaking liquid and soaking residue, centrifuge the soaking liquid, and use ultrafiltration equipment for the centrifuged supernatant (molecular weight cut off is greater than 1000) Filtrate, soak the clear solution in water, add 15 times the volume concentration of 50% ethanol to the leaching residue, reflux extraction in a water bath at 70°C for 3 times, extract for 1 hour each time, combine the extract solution, add alkali to the extract solution to adjust pH=8, and carry out vacuum Concentrate, the concentration temperature is 70°C, concentrate until there is no alcohol smell, recover ethanol, and centrifuge to obtain concentrated supernatant;

[0032] (2) Concentrated supernatant and water-soaked supernata...

Embodiment 3

[0034] Embodiment three of the present invention is: a kind of extracting method of sapindus saponin, comprises the following steps:

[0035] (1) Take 100g of sapindus fruit peel raw material, add 6 times the volume of water to soak 3 times, 3 hours each time, collect all the soaking liquid and soaking residue, centrifuge the soaking liquid, and use ultrafiltration equipment for the centrifuged clear liquid (molecular weight cut off is greater than 1000) Filtrate, soak the clear solution in water, add 12 times the volume concentration of 70% ethanol to the soaked residue, and extract twice in a water bath at 80°C under reflux for 1.5 hours each time, combine the extracts, add alkali to the extracts to adjust pH=9, and carry out Concentrate in vacuo, the concentration temperature is 65°C, concentrate until there is no alcohol smell, recover ethanol, centrifuge to obtain concentrated supernatant;

[0036] (2) Concentrated supernatant and water-soaked supernatant are merged, and ...

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Abstract

The invention discloses a method for extracting Sapindus saponins, which comprises the following steps: S1. After taking Sapindus pericarp and immersing in water for several times, collecting all the soaking liquid and soaking residue respectively, and extracting the part with a molecular weight greater than 1000 in the soaking liquid After removal, the water-soaked clear liquid is obtained; after the leaching residue is extracted with alcohol, the extracts are combined and concentrated to obtain a concentrated clear liquid; S2, the water-soaked clear liquid and the concentrated clear liquid are combined, and purified through a non-polar macroporous resin, Then eluted with pure water and three ethanol solutions with different concentrations in sequence; S3, collecting the second and third eluents for concentration, and drying the concentrates respectively to obtain powder I and powder II, wherein the powder I is echinocystic acid type saponin, powder II is oleanolic acid type saponin. The sapindus saponin product obtained by adopting the elution gradient of the scheme of the present invention has high purity, high yield, light color and off-white color, and the scheme of the present invention does not need to use toxic reagents and is environmentally friendly.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to a method for extracting Sapindus saponins. Background technique [0002] Sapindus belongs to Sapindus family Sapindus, scientific name: Sapindus Mukurossi Gaertn, Compendium of Materia Medica is called Mukurossi Gaertn, Sichuan is called Oil Sapindus, Hainan Island is called Sapindus Mukurossi Gaertn, and Taiwan is called Sapindus Mukurossi Gaertn. It is Huangmuzi, also known as oil luoshu, washing fruit, soap fruit tree and so on. Sapindus saponin is the extract of Sapindus pericarp, and its main active ingredients are triterpene saponins, sepinene glycosides, fatty oil and protein. According to different aglycones, triterpene saponins can be divided into oleanolic acid saponins and echinocystic acid saponins, etc. According to research reports, oleanolic acid saponins are effective in antitumor, hypoglycemic, hypolipidemic, anti-inflammatory and antibacterial, and ant...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00
CPCC07J63/008
Inventor 宁丹贺玉婷易宇阳康军曹惠璋
Owner CHANGSHA LANGLIN BIOLOGY PROD