Anti-cancer hydrogel composition and preparation method thereof

A composition and hydrogel technology, applied in the field of medicine, can solve problems such as weak therapeutic effect, and achieve the effects of economical price, regulation of T cell activity, and prolongation of drug action time.

Pending Publication Date: 2020-07-03
大连市慧恩生物科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the therapeutic effect of hypothermia and hyperthermia is weak, requiring multiple or continuous photothermal treatments induced by photothermal agents, and usually needs to be combined with other therapies to accurately and effectively destroy tumors

Method used

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  • Anti-cancer hydrogel composition and preparation method thereof
  • Anti-cancer hydrogel composition and preparation method thereof
  • Anti-cancer hydrogel composition and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A preparation method of an anti-cancer hydrogel composition, specifically according to the following steps:

[0044] S1, calculate the mass ratio according to the final concentration of each component, weigh sodium alginate, ink, azobisisobutylimidazoline hydrochloride, PD-1 targeting or PD-L1 targeting antibody, chemotherapy drugs, Soluble in ddH 2 O, ultrasonically dissolved to prepare the corresponding aqueous solution;

[0045] Add 20mg sodium alginate to 1mLddH 2 In O, ultrasonically dissolve to prepare 20mg / mL sodium alginate aqueous solution; take 5mg of conventional ink and dissolve it in 5mlddH 2 In O, use the atomic layer deposition (ALD) technology to process the dissolved ink solution, and prepare a 1mg / ml ink (INK) aqueous solution, which has broad-spectrum absorption characteristics in the range of 300-1200nm, and generates light through NIR-II laser irradiation. Thermal effect; take 0.75mg azobisisobutylimidazoline hydrochloride (AIPH) and add it to 1m...

Embodiment 2

[0049] A preparation method of an anti-cancer hydrogel composition, specifically according to the following steps:

[0050] S1, calculate the mass ratio according to the final concentration of each component, weigh sodium alginate, ink, azobisisobutylimidazoline hydrochloride, PD-1 targeting or PD-L1 targeting antibody, chemotherapy drugs, Soluble in ddH 2 O, ultrasonically dissolved to prepare the corresponding aqueous solution;

[0051] S2. At room temperature, take AIPH aqueous solution, PD-1-targeting or PD-L1-targeting antibody aqueous solution, and chemotherapeutic drug aqueous solution, mix them, and ultrasonically mix the solutions in a water bath with a constant temperature of 55°C to make the solutions evenly mixed and uniformly improve drug dissolution. temperature, add sodium alginate aqueous solution and ink aqueous solution to the mixed solution after mixing, fully shake and mix, and prepare 2ml final concentration of sodium alginate 3mg / mL, ink 23μg / mL, azobisi...

Embodiment 3

[0054] A preparation method of an anti-cancer hydrogel composition, specifically according to the following steps:

[0055] S1, calculate the mass ratio according to the final concentration of each component, weigh sodium alginate, ink, azobisisobutylimidazoline hydrochloride, PD-1 targeting or PD-L1 targeting antibody, chemotherapy drugs, Soluble in ddH 2 O, ultrasonically dissolved to prepare the corresponding aqueous solution;

[0056] S2. At room temperature, take AIPH aqueous solution, PD-1-targeting or PD-L1-targeting antibody aqueous solution, and chemotherapeutic drug aqueous solution, mix them, and ultrasonically mix the solutions in a water bath with a constant temperature of 55°C to make the solutions evenly mixed and uniformly improve drug dissolution. temperature, add sodium alginate aqueous solution and ink aqueous solution to the mixed solution after mixing, fully shake and mix, and prepare 2ml final concentration of sodium alginate 4.5mg / mL, ink 25μg / mL, azobi...

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Abstract

The invention discloses an anti-cancer hydrogel composition and a preparation method thereof. The anti-cancer hydrogel composition is composed of the following components according to the final concentration: 3.0-4.5 mg / mL sodium alginate, 20-25 [mu]g / mL myrrh, 135-195 [mu]g / mL 2,2'-azobis[2-(imidazolin-2-yl)propane]dihydrochloride, a 150-550 [mu]g / mL PD-1 targeting or PD-L1 targeting antibody, and a 100-150 [mu]g / mL chemotherapy drug. The anti-cancer hydrogel composition provided by the invention can improve the tumor immune microenvironment under low-temperature light and heat, regulate theactivity of T cells, activate innate immunity and adaptive immunity in vivo, inhibit the growth of tumor in situ, inhibit tumor distant metastasis, and enhance the anti-tumor effect, and has minimal side effects, and excellent biocompatibility and degradability.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to an anticancer hydrogel composition and a preparation method thereof. Background technique [0002] Colorectal cancer is a malignant tumor with high morbidity and mortality. According to the data of "Cancer Statistics, 2019" published by CA magazine, there will be 145,000 new cases and 51,000 deaths in the United States in 2019. The death cases occupy the third place among the malignant tumors in the United States. In addition, according to the "Analysis of the Epidemic Situation of Malignant Tumors in China in 2015" released by the China Cancer Center in 2019, among the top 10 malignant tumors, colorectal cancer ranks third among new cancer cases in both men and women in China, and the number of people is even higher. The number reached 388,000, and the number of deaths reached 187,000. Although colorectal cancer can be treated with multiple treatments such as surgery, chemother...

Claims

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Application Information

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IPC IPC(8): A61K9/06A61K47/36A61K41/00A61K39/395A61K31/555A61K31/704A61P35/00
CPCA61K9/06A61K47/36A61K41/0057A61K41/0052A61K39/39558A61K31/555A61K31/704A61P35/00A61K2300/00Y02A50/30
Inventor郭慧淑沈顺庞志清蔡正旭宁波
Owner大连市慧恩生物科技开发有限公司