The invention relates to the technical field of microbial
probiotic preparations, in particular to a
sodium alginate multifunctional composite
microsphere as well as a preparation method and application thereof. The
sodium alginate multifunctional composite microspheres are obtained by dissolving the
carbohydrate vesicles, the
traditional Chinese medicine nanoparticles and the probiotics in a
sodium alginate solution and then dropwise adding the solution into a
calcium chloride solution through an ionic cross-linking effect. According to the invention, the amphiphilic
xylooligosaccharide-
cystamine derivative with H2S response is synthesized through a click reaction on the basis of
prebiotic xylooligosaccharide. And the amphipathic saccharide vesicles are obtained through self-
assembly by virtue of hydrophilic and hydrophobic effects.
Sanguinarine iodide prepared by purification, separation and
reflux and
baicalin are self-assembled in an
aqueous solution through a non-
covalent bond, and the
traditional Chinese medicine nanoparticles are prepared through ultrasonic treatment. An in-vitro simulated release experiment proves that the
sodium alginate multifunctional composite
microsphere is relatively low in release rate under an acidic condition, so that substances are favorably delivered to intestinal tracts; and the release of encapsulated substances can be better realized in neutral and alkaline environments.