Process for optimally extracting quercetin from perfoliote knotweed herb by using Plackett-Burnman combined response surface method

Abstract

The invention discloses a process for optimally extracting quercetin from perfoliote knotweed herb by using a Plackett-Burnman combined response surface method. According to the process, the extraction amount of quercetin in perfoliote knotweed herb is taken as an investigation index, and extraction research is performed by adopting the Plackett-Burnman combined response surface method, so the optimal extraction process for quercetin in perfoliote knotweed herb is obtained; i.e., the perfoliote knotweed herb is soaked in ethanol with a concentration of 59.92% and accounting for 25 times of thevolume of the perfoliote knotweed herb for 24 hours, and then reflux extraction is conducted for 3.4 hours twice; under the condition of the extraction process, the average extraction amount of quercetin is 0.34 mg/g. An extract prepared by optimizing the extraction process is high in antibacterial activity, the MIC of the extract to escherichia coli, staphylococcus aureus and salmonella is 3.12g/L, 6.24 g/L and 6.31 g/L respectively, and the MBC of the extract to escherichia coli, staphylococcus aureus and salmonella is 12.52 g/L, 24.08 g/L and 25.41 g/L respectively; and the quercetin canbe used for treating livestock and poultry bacterial diseases caused by escherichia coli, staphylococcus aureus and salmonella.

Description

technical field [0001] The invention relates to the technical field of extraction of traditional Chinese medicines, in particular to a method for optimizing the extraction of quercetin from sarcocarpus by combining Plackett-Burnman with response surface method. Background technique [0002] Perfoliatum is the whole herb of Polygonum perfoliatum L., which is a plant of the genus Polygonum perfoliatum. It is commonly known as snake back, cat's claw thorn, perfoliatum, etc. Its main components include flavonoids, organic acids, and terpenes. Wait. The second part of the "Chinese Veterinary Pharmacopoeia" 2015 edition stipulates that quercetin is the main component of Ganbangui. Modern pharmacological studies have shown that Ganbangui has antibacterial, antiviral, blood pressure-lowering, and anti-tumor effects. Clinically, Ganbangui is often used to treat chronic eczema, mastitis, whooping cough, diarrhea, jaundice, bruises, swelling and pain, etc. . The decoction of Ganbang...

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Problems solved by technology

[0007] The above literature uses the orthogonal design method to optimize the extraction of the effective components of the bar plate. The orthogonal design method can consider mult

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