Subunit F protein of peste des petits ruminants virus as well as preparation method and application of subunit F protein

Abstract

The invention belongs to the technical fields of animal vaccines and veterinary biological products, and particularly relates to a subunit F protein of a peste des petits ruminants virus as well as apreparation method and application of the subunit F protein. An amino acid sequence of the subunit F protein is as follows: (1) an amino acid sequence represented by SEQ ID NO.1; (2) a derived immunogenic amino acid sequence obtained from the amino acid sequence represented by SEQ ID NO.1 through substitution, deletion and addition of one or more amino acids. The subunit F protein is mainly prepared by the steps of constructing recombinant plasmids, transfecting cell strains by virtue of the recombinant plasmids, screening highly-expressed cell strains, and purifying the subunit F protein of the peste des petits ruminants virus. The subunit F protein is well applied to subunit vaccines or diagnostic reagents for the peste des petits ruminants and has the characteristics that secretory expression is efficient, the protein purity is high, purification is easily realized, the production cost is lowered, the safety performance is high, and the like.

Description

technical field [0001] The invention belongs to the technical field of animal vaccines and veterinary biological products, and in particular relates to subunit F protein of Peste des Petits Ruminants virus and its preparation method and application. Background technique [0002] Peste des Petits of Ruminats (PPR) is a highly contagious viral disease caused by Peste des Petits of Ruminats Virus (PPRV). The World Organization for Animal Health (OIE) classifies it as a Class A infectious disease, and my country also classifies it as a Class I epidemic disease. PPRV mainly infects sheep, especially goats are highly susceptible. Once infected sheep will be characterized by sudden fever, depression, oral ulcers, cough, eye and nose discharge, and diarrhea. The morbidity and mortality can reach 100%, causing huge economic losses and seriously affecting the development of the sheep industry. [0003] At present, there is no effective treatment for the disease, mainly relying on va...

AI Technical Summary

Problems solved by technology

However, subunit vaccines also have obvious shortcomings: high production costs and limited applications

[0007] However, when engineered cells are used to express F protein, if the coding gene sequence of F protein is not optimized, engineered cells basically do not express F protein, and it is difficult to obtain F protein with excellent immunogenicity and stability for small ruminant The prevention and control of zooepidemic virus, therefore, when using engineered cells to express F protein, optimizing the coding gene sequence of F protein is a necessary process

Images