Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Culture method for acid-tolerant alpha-amylase strains

A culture method and amylase technology, which are applied in biochemical equipment and methods, enzymology/microbiology devices, bioreactors/fermenters for specific purposes, etc., can solve the problem of slow heating area, uneven temperature, and temperature rise speed problem

Inactive Publication Date: 2020-07-07
叶绪权
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] It needs to be cultivated on a shaker at 30°C and 150r / min for 24 hours. The traditional shaker device uses heating lamps or heating wires to heat the air in the incubator to achieve the effect of heat preservation. Heating will easily cause the temperature in this area to rise faster. Other areas The heating area is relatively slow, which can easily cause uneven temperature in the two areas and affect the quality of culture

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Culture method for acid-tolerant alpha-amylase strains
  • Culture method for acid-tolerant alpha-amylase strains
  • Culture method for acid-tolerant alpha-amylase strains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0023] Please refer to figure 1 , figure 2 , image 3 and Figure 4 , a method for cultivating acid-resistant α-amylase strains, comprising the following steps:

[0024] s1, preparing medium for acid-resistant α-amylase strains;

[0025] s2, planting the acid-resistant α-amylase strain into the medium in s1 by streaking on a plate to form a petri dish;

[0026] s3, put the culture dish prepared in s2 into a special centrifugal homogeneous incubator for cultivation;

[0027] The special centrifugal uniform temperature incubator includes an incubator 1, a shaking structure 2, a petri dish storage heating structure 3 and a shock-absorbing support structure 4, the petri dish storage heating structure 3 is connected to the incubator 1, and the petri dish storage heating structure 3 Including drawing frame 31, cover plate 32, holding plate 33, sealing ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of amylase strain culture, in particular to a culture method for acid-tolerant alpha-amylase strains. The culture method comprises the following steps of s1, preparing an acid-tolerant alpha-amylase strain culture medium; s2, planting acid-tolerant alpha-amylase strains into the culture medium in the step s1 by adopting a plate streak method to form culture dishes; and s3, putting the culture dishes prepared in the step s2 into a special centrifugal uniform-temperature incubator for culture. The special centrifugal uniform-temperature incubator provided by the invention has the advantages that heating is carried out in a water bath manner, and uniform temperature of a storage environment of each culture dish is ensured, so that the culture quality is ensured, the number of the culture dishes is relatively large and the culture efficiency is improved.

Description

technical field [0001] The invention relates to the field of cultivating amylase strains, in particular to a method for cultivating acid-resistant alpha-amylase strains. Background technique [0002] The re-screening process for the production of acid-resistant α-amylase strains: Inoculate the first-screened strains into the seed medium, and cultivate them on a shaking table at 30°C and 150r / min for 24h. Take 1 mL of the solid-state enzyme-producing medium, culture it at 30°C for 72 hours, and measure the activity of α-amylase, and store the strain with the best activity at 4°C. [0003] It needs to be cultivated on a shaker at 30°C and 150r / min for 24 hours. The traditional shaker device uses heating lamps or heating wires to heat the air in the incubator to achieve the effect of heat preservation. Heating will easily cause the temperature in this area to rise faster. Other areas The heating area is relatively slow, and it is easy to cause uneven temperature in the two are...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12M1/38C12M1/00
CPCC12M27/16C12M41/22C12N1/00
Inventor 叶绪权谢佳伟王旭
Owner 叶绪权
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com