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Novel artificial skin and preparation method thereof

A kind of artificial skin, a new type of technology, applied in the field of tissue engineering biomaterials, can solve the problems of unfavorable clinical use of products, increase product safety hazards, difficult to remove reagents, etc., achieve excellent decellularization effect, promote rapid growth and reproduction, and promote growth. Effect

Inactive Publication Date: 2020-07-14
山东隽秀生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the organic solvents used in the degreasing step in the method, such as n-hexane and isopropanol, are toxic, and acetone is more prone to poisonous chemicals. Although the degreasing treatment is relatively perfect, the reagent residue is difficult to remove, which increases product safety hazards and is not conducive to subsequent products. clinical use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Take 500 g of fresh isolated porcine head skin, add 1000 mL of 0.5 mol / L sodium hydroxide solution to soak for 10 hours, rinse with deionized water, and drain the water. Scrape excess body hair and subcutaneous adipose tissue, cut the obtained dermis into small pieces of 0.3 cm, shake and rinse with deionized water 5 times, each time for 20 minutes;

[0033] (2) Shake with 60U / mL DNase, 0.25% w / v trypsin solution (containing 0.1% w / v EDTA) for 2 hours, 0.3% sodium dodecyl sulfate for 1.5 hours, and 0.4% peracetic acid The solution was shaken for 1 hour, and the PBS solution was shaken for 1 hour. After each step, it was rinsed with deionized water and drained. Freeze-drying the obtained acellular dermal matrix;

[0034] (3) 20 g of the acellular dermal matrix prepared in step (2) was dissolved in 400 mL of pepsin solution with a concentration of 0.5 mg / mL, and shaken at 37° C. for 25 hours to obtain an acellular dermal matrix solution. While oscillating, add an ap...

Embodiment 2

[0039] (1) Take 600g of fresh isolated rabbit skin, add 1200mL of 0.05mol / L sodium hydroxide solution to soak for 6h, rinse with deionized water, and drain the water. Scrape off excess body hair and subcutaneous adipose tissue, cut the obtained dermis into small pieces of 0.5 cm, shake and rinse with deionized water 3 times, each time for 10 minutes;

[0040] (2) Shake with 40U / mL DNase, 0.1% w / v trypsin solution (containing 0.05% w / v EDTA) for 1 hour, 0.2% sodium dodecyl sulfate for 1 hour, and 0.2% peracetic acid Treat for 1 hour, shake the PBS solution for 1 hour, rinse with deionized water at the end of each step, and drain the water. Freeze-drying the obtained acellular dermal matrix;

[0041] (3) Dissolve 40 g of the acellular dermal matrix prepared in step (2) in 800 mL of pepsin solution with a concentration of 0.2 mg / mL, and shake at 37° C. for 22 hours to obtain an acellular dermal matrix solution. While oscillating, add an appropriate amount of sodium hydroxide so...

Embodiment 3

[0046] (1) Take 18 adult mice grown in the same environment, divided into 3 groups, named experimental group 1, experimental group 2 and control group respectively and numbered, and used for the experiment after adaptive feeding for 1 week;

[0047] (2) 18 mice were shaved at the same position on the back, and artificially caused a small area of ​​superficial skin defect trauma after disinfection;

[0048] (3) The 6 mice in experimental group 1 were divided into two groups. The 3 mice in group A used the pig-derived artificial skin prepared in Example 1 to repair the wound, and soaked the artificial skin material with a small amount of mouse blood before use; the 3 mice in group B Repair the wound with the pig-derived artificial skin prepared in Example 1, soak the artificial skin material with a small amount of normal saline before use;

[0049] (4) The 6 mice in the experimental group 2 were divided into two groups. The 3 mice in the C group used the rabbit-derived artificia...

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Abstract

The invention provides a novel artificial skin and a preparation method thereof. The preparation method of the novel artificial skin comprises the following specific steps of taking fresh in-vitro animal skin, carrying out a series of treatment of sodium hydroxide, deoxyribonuclease, trypsase, sodium lauryl sulfate, peroxyacetic acid, PBS and the like and then obtaining an acellular dermal matrix;mixing graphene oxide with the acellular dermal matrix evenly through ultrasonic mixing; and then carrying out enrichment treatment to obtain the novel artificial skin. The acellular dermal matrix with uniform and stable components can be produced, the physical strength of the matrix is improved through addition of the graphene oxide; the enrichment treatment provides an excellent environment foradhesion, crawling and growth of epidermal stem cells; and the novel artificial skin has a significant effect on wound healing. Through the application of the novel artificial skin, invasion and destruction of bacteria to an impaired wound can be prevented, wound infection can be prevented, differentiation of the stem cells into skin cells can be selectively induced, regeneration of wound skin ispromoted and the transplant success rate is improved.

Description

technical field [0001] The invention belongs to the field of tissue engineering biomaterials, and in particular relates to a novel artificial skin and a preparation method thereof. Background technique [0002] Skin is an important tissue and organ that covers and protects the body surface. Skin defects and abnormalities caused by inflammation, ulcers, burns, congenital deformities and other reasons are common clinical phenomena. For this kind of tissue defect, the method of autologous skin transplantation is mostly used at present, but the source of autologous skin is extremely limited and it will cause secondary damage to the patient when the material is taken, which will affect the treatment process. In view of this, tissue engineered skin is the most potential treatment method, and acellular dermal matrix, as a natural extracellular matrix material, has good mechanical properties, histocompatibility and low immunogenicity, and is suitable for tissue It is a good matrix ...

Claims

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Application Information

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IPC IPC(8): A61L27/60A61L27/36A61L27/08A61L27/50
CPCA61L27/08A61L27/362A61L27/3687A61L27/3691A61L27/50A61L27/60A61L2430/40
Inventor 高秀岩曲梁静姜红
Owner 山东隽秀生物科技股份有限公司
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