Colony counting method based on machine vision

A technology of colony counting and machine vision, which is applied in the field of colony counting based on machine vision, can solve problems such as low counting efficiency, different counting results, and easy interference from the external environment during the dotting process, and achieve the effect of improving efficiency and accuracy

Pending Publication Date: 2020-07-17
马健锦
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Traditionally, for the counting of colonies, the method of manually dotting the lid of the petri dish with a marker pen is used, and then the statistics are performed after the dots are completed. The traditional method is easy to cause fatigue, and the dot process is easily disturbed by the external environme

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  • Colony counting method based on machine vision
  • Colony counting method based on machine vision

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Embodiment Construction

[0037] The general idea of ​​the technical solution in the embodiment of the present application is as follows: perform preprocessing on the obtained original photos to reduce noise, which is convenient for later binarization processing and contour traversal; perform preprocessing on the optimized photos generated after preprocessing The second traversal, the first traversal finds the first outline of the petri dish, and sets the area outside the first outline to black to reduce interference and reduce the amount of calculation, and the second traversal finds the petri dish For the second contour of the colony in the photo, the number of the second contour is counted by counting function to count the colony.

[0038] Please refer to Figure 1 to Figure 2 Shown, a kind of preferred embodiment of the colony counting method based on machine vision of the present invention, comprises the steps:

[0039] Step S10, obtaining the original photo of the petri dish, and performing prep...

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Abstract

The invention provides a bacterial colony counting method based on machine vision in the field of bacterial colony counting, which comprises the following steps: step S10, acquiring an original photoof a culture dish, and preprocessing the original photo to generate an optimized photo; S20, traversing and searching a first outline of a culture dish in the optimized photo, setting an area outsidethe first outline to be black, and generating a photograph of the culture dish; S30, traversing and searching a second outline of each bacterial colony in the photograph; and step S40, counting the second contours. The bacterial colony counting device has the advantage that the bacterial colony counting efficiency and precision are greatly improved.

Description

technical field [0001] The invention relates to the field of colony counting, in particular to a method for counting colonies based on machine vision. Background technique [0002] The determination of the total number of bacterial colonies is used to determine the degree of food contamination by bacteria and the hygienic quality. It reflects whether the food meets the hygienic requirements during the production process, so as to make an appropriate hygienic evaluation of the tested samples. The total number of bacterial colonies can be determined to a certain extent. It marks the quality of food hygiene. In order to count the colonies, the sample to be tested needs to be diluted first, and then poured on a petri dish for cultivation, and the colonies are counted after culturing for a certain period of time. [0003] Traditionally, for the counting of colonies, the method of manually dotting the lid of the petri dish with a marker pen is used, and then the statistics are pe...

Claims

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Application Information

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IPC IPC(8): G06T5/00G06T7/00G06T7/136G06T7/90G06M11/02
CPCG06T5/002G06T7/0012G06T7/136G06T7/90G06M11/02G06T2207/30242G06T2207/20028
Inventor 马健锦
Owner 马健锦
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