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Time-resolved fluorescence measuring system based on multichannel TCSPC and multiple detectors

A technology for time-resolved fluorescence and measurement systems, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of low counter utilization and weak fluorescence, achieve fast fluorescence lifetime imaging, and improve counting efficiency

Active Publication Date: 2018-01-30
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the traditional TCSPC system requires that the excited fluorescence is weak enough for each excitation, and can only record less than 0.01 photons per excitation cycle. Only one photon per excitation cycle reaches the counter, so the utilization of the counter is not high

Method used

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  • Time-resolved fluorescence measuring system based on multichannel TCSPC and multiple detectors
  • Time-resolved fluorescence measuring system based on multichannel TCSPC and multiple detectors
  • Time-resolved fluorescence measuring system based on multichannel TCSPC and multiple detectors

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Embodiment 1

[0020] Such as figure 2 As shown, a time-resolved fluorescence spectroscopy measurement system based on a few-channel time-correlated single-photon counter and multiple detectors according to an embodiment of the present invention includes: a laser light source 1, a spectroscopic component 2, a plurality of single-photon detectors 3, A time-correlated single photon counter 4 and a computer 5 .

[0021] The laser light source 1 is used to emit high-frequency pulsed laser to excite the sample to emit fluorescence. The sample is placed in the sample chamber. The fluorescence emitted by the sample reaches the spectroscopic assembly 2 through the lens, where the main function of the lens is to focus the fluorescence onto the detector. The light splitting component 2 is used to separate the fluorescent light of different wavelengths in space. The light splitting component can be a dispersion element such as a grating or a prism. A plurality of single-photon detectors 3 include ...

Embodiment 2

[0041] The solution of Embodiment 2 of the present invention will be introduced below. Here, only the parts different from Embodiment 1 will be described in detail, and the same parts will not be described again. Generally, when the number of photon detectors is small, the number of channels of the single photon counter may only have two channels, the first channel and the second channel, except the zeroth channel ch0. In this case, set the connecting cables between each photon detector to equal length L, such as Figure 5 shown.

[0042] In this embodiment, different from Embodiment 1, the arrival time measured by the first channel is determined according to the time difference between the photoelectric pulse signal of a certain photon detector arriving at the first channel and the second channel of the single photon counter Corresponding photon detector number.

[0043] In this scheme, since the connection lines (cables) between each detector are preset to be equal in leng...

Embodiment 3

[0047] The solution of Embodiment 3 of the present invention will be introduced below. Here, only the parts different from Embodiment 1 will be described in detail, and the same parts will not be described again. Such as Figure 6 As shown, in the case that the number of photon detectors is large, the number of channels of the single photon counter can have the first channel, the second channel, the third channel and the fourth channel except the zeroth channel, and the multiple The spatial positions of the photon detectors are set as a two-dimensional array, and each detector is connected to the cable a and the cable b in a grid-like manner. The two ends of cable a are respectively connected to ch1 and ch2 of the single photon counter, and the two ends of cable b are respectively connected to ch3 and ch4 of the single photon counter. Any detector in the array receives a photon, and its signal will be transmitted to four channels ch1, ch2, ch3 and ch4, corresponding to four t...

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Abstract

The invention provides a time-resolved fluorescence measuring system based on a multichannel TCSPC and multiple detectors. The time-resolved fluorescence measuring system comprises a laser light source, a beam split component, the plurality of photon detectors and the single photon counter, wherein the laser light source is used for emitting high-frequency pulse laser to excite a sample to emit fluorescence; the beam split component is used for splitting the fluorescence of different wavelengths in a space; the photon detectors are arranged at different positions; the single photon counter hasmore than two channels. The time-resolved fluorescence measuring system has the advantages that the photoelectric pulse signal detected by each photon detector is transmitted to the multiple channelsof the single photon counter through different circuits connecting the detectors, and the number of the photon detectors is determined according to the time, reaching the different channels, of photoelectric pulse signals; the system uses the multiple detectors, and the utilization efficiency of the counters can be greatly increased to reach the maximum.

Description

technical field [0001] The invention relates to the technical field of time-resolved fluorescence measurement, in particular to a time-resolved fluorescence system based on a few-channel time-correlated single photon counter (TCSPC) and a detector. Background technique [0002] Time-correlated single-photon counting (TCSPC, time-correlated single-photon counting) technology was established in the 1960s to detect the luminescence of scintillators excited by rays, and later it was applied to the measurement of fluorescence lifetime. It has the advantages of good time resolution, high sensitivity, high measurement accuracy, large dynamic range, digital output data, and convenient computer storage and processing. The structure and basic working principle of the TCSPC time-resolved fluorescence measurement system (hereinafter referred to as "TCSPC system"), such as figure 1 As shown in (a), the TCSPC system usually uses a high repetition frequency excitation light source. Now co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 张三军李磊吴光
Owner EAST CHINA NORMAL UNIV
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