Microcarrier suitable for cell growth and method for culturing microcarrier in microenvironment

Abstract

The invention discloses a microcarrier suitable for cell growth and a method for culturing the microcarrier in a microenvironment. The microcarrier comprises a plurality of cells, a carrying solutionused for carrying the plurality of cells and mixing the cells to form a plurality of inner cores, and a shell layer, the shell layer is alginic acid and coated at the periphery of each inner core, thealginic acid and a calcium chloride solution are mixed for mild crosslinking to form a plurality of porous micro-carriers, the porous micro-carriers are placed in a culture medium through the carrying solution with enhanced cell attachment capability, culture is performed in an environment of 37 DEG C and 5% CO2 to promote cells to form cell masses, and the shell layer is removed in a calcium ionremoval mode to obtain pure cell masses.

Description

technical field [0001] The invention relates to a microcarrier suitable for cell growth and a method for culturing the microcarrier in a microenvironment, in particular to a capsule cell carrier, which uses biodegradable materials and can be easily regenerated after the cells are cultured into clumps. The outer material of the agglomerate is carefully removed to obtain a cell agglomerate with a cell matrix and to apply it. Background technique [0002] At present, the cell culture system accounts for a large part of the contribution in self-cell therapy, which can avoid large-scale and high-cost animal experiment resources. However, the traditional cell culture method makes cells grow in a single layer, which leads to gradual The original characteristics of cells, such as cell type or protein secretion, are lost, so they appear different from those in vivo, which affects the expected results of subsequent animal experiments or human experiments. Therefore, the 3D cell cultur...

AI Technical Summary

Problems solved by technology

Now we can also see that the 3D cell culture system is expected to grow at a compound annual growth rate of 8.7% in the future. The current 3D culture system has the following three models, but they still have their own disadvantages: 1. Hanging drop (hanging drop), unable to grow larger cell clusters; 2. cell scaffold (scaffold), will cause a large number of cells to lose, and the removal of materials is quite difficult; 3. Hydrogel system (hydrogel system), is the permeability of materials Therefore, the above three modes are not conducive to the growth of cells

[0004] However, most traditional three-dimensional cell cultures use polymer materials to prepare scaffolds or carriers, which have poor effects on cell growth and attachment. In addition, after forming cell clumps in carriers or scaffolds, they are made of polymer materials. The carrier is not easy to remove, and cannot be effectively separated from the cell mass. The cell mass and the carrier must be implanted into the affected area together, resulting in poor connection between the cell mass and the cells near the affected area and poor cell integration, thereby reducing the effect of cell therapy.

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