The construction and application of single-core, two-core and mature corn pollen separation methods and pollen development stage judgment model
A technology of corn pollen and development stage, applied in the field of plant biochemistry, can solve the problems of tassel destruction, poor accuracy of pollen cell stage judgment, complicated operation, etc., and achieve the effect of maintaining activity, non-toxic density, and simple operation
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Embodiment 1
[0045] The separation and purification method of maize mononuclear, dinuclear and mature pollen, see figure 1 , including the following steps:
[0046] (1) According to the number of leaf rings and the length of spikelets, the development stage of the pollen can be roughly judged, and the corn tassels are taken (for early tassels that are still wrapped in the top leaves, the corn tassels wrapped in leaves can be carefully dissected. ), put them in a ziplock bag and store them on ice, and according to the length of the spikelets on the tassels, take the spikelets near the middle of the tassels, put them into a juice extractor, add 4°C pre-cooled grinding buffer, and grind for 12 ~15 s (slightly oscillate up and down for grinding, pause once every 3s, and grind 4 times);
[0047] (2) Use different mesh sieves (if you want to separate and purify mononuclear pollen, choose 300 mesh sieve, use 230 mesh sieve when separating dinuclear pollen, use 150 mesh sieve when separating matu...
Embodiment 2
[0055] Experimental Study on the Correlation Between Leaf Ring Number, Spikelet Length on Tassel, Pollen Diameter and Pollen Development Stage in Maize Plants
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