Polarization Imaging Detection Method for Cells on Undewaxed-Non-stained Sections

Abstract

The invention discloses a method for detecting polarization imaging of cells on non-dewaxing-non-stained slices, belonging to the technical field of polarization measurement; it is characterized in utilizing high-resolution, high-fidelity and high-precision cell Mueller imaging detection technology and method; the detection method It is to first obtain the Mueller image at the cell level, and then determine the position distribution of paraffin on the slice from the phase delay image processed by Mueller pole decomposition, and then remove the influence of paraffin in a series of images after Mueller decomposition, and obtain the true polarization of the cell information. A series of Mueller decomposition images after removing the influence of paraffin can not only truly reflect the cell structure and arrangement characteristics, but also provide rich information on the internal structure of cells. This method has the advantages of no need for staining of sliced ​​samples, high fidelity of cell arrangement and structure, and the ability to obtain rich information on the internal structure of cells, which effectively helps the rapid and accurate diagnosis of cancer.

Description

Technical field[0001]The present invention belongs to the technical field of polarization measurement, and more particularly to a polarization imaging detection method of unwaffene-non-stained sections.Background technique[0002]Cancer is an important reason why the human death rate is high. In numerous cancer testing methods, histopathological slices observations are the most accurate and reliable method of diagnosis of tumors, known as the gold standard for cancer diagnosis. Pathological diagnosis is a pathological observation of tissue sections to tissue sections by optical microscopy, and analyzes the morphological structure and distribution of experienced pathologists to achieve cancer assessment. The tissue sections observed by pathology usually need to be stained with dyes (Sumu and Iraqi, etc.). When the actual dyeing process is cumbersome, the dewaxing and dyeing process can change the true structure of cells varying degrees to different degrees, which may affect the correct...

AI Technical Summary

Problems solved by technology

However, the existing polarization detection uses a low numerical aperture objective lens (low resolution), which can only detect Mueller imaging at the tissue level, and cannot perform accurate quantitative diagnosis of cancer