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Method for determining content of 10-DAB III in taxus chinensis branches and leaves

A technology for the determination of 10-DABⅢ content in yew branches and leaves, which can solve the problems of short analysis time and achieve the effects of saving analysis time, stable baseline, and improved resolution and peak shape.

Inactive Publication Date: 2020-08-07
SHANGHAI PUTUO DISTRICT CENT HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on a method for the determination of 10-DABⅢ content in yew branches and leaves with short analysis time, simple sample pretreatment method and good separation effect.

Method used

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  • Method for determining content of 10-DAB III in taxus chinensis branches and leaves
  • Method for determining content of 10-DAB III in taxus chinensis branches and leaves
  • Method for determining content of 10-DAB III in taxus chinensis branches and leaves

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Experimental program
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Embodiment 1

[0016] 1 material

[0017] Instrument: Waters ACQIUTY UPLC H CLASS; ultrasonic cleaner; electronic balance;

[0018] Materials: 10-DabⅢ reference substance, southern yew leaf powder (passed through a 18-mesh sieve); acetonitrile, formic acid (chromatographically pure, Thremo); ultrapure water, and other analytical reagents were purchased from Sinopharm.

[0019] 2 For methods and results, see figure 1 , figure 2 and image 3 .

[0020] 2.1 Preparation of reference solution

[0021] Accurately weigh 12.18mg of 10-DabⅢ reference substance and place it in a 20mL volumetric flask, add methanol to the volume and mix well to prepare a 0.609mg / mL standard solution, and dilute to 0.3045, 0.1523, 0.762, 0.381, 0.191 mg / mL.

[0022] 2.2 Chromatographic conditions and system adaptability test

[0023] Chromatographic column: Acquity BEH Phenyl (2.1×100mm, 1.7μm) Mobile phase: acetonitrile (A): water (B), gradient elution conditions: 0-0.3 15% A, 0.3-5.0 22% A, 5.0-6.0 95 %A,6.0-...

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Abstract

The invention relates to a method for determining the content of 10-DAB III in taxus chinensis branches and leaves, the method adopts a water-acetone (50: 50) mixed solvent for ultrasonic extraction,the method adopts a 2.1 * 100mm and 1.7 [mu]m phenyl column, and the flow velocity of the method is 0.3-0.6 mL / min. The pretreatment method for determining the content of 10-DAB III in taxus chinensisbranches and leaves has the advantages that a water-acetone (50: 50) mixed solvent is adopted for ultrasonic extraction, extraction of effective components can be guaranteed, meanwhile, impurities are few, a base line is stable, and the separation degree and the peak shape are remarkably improved. The established ultra-high performance liquid chromatography UHPLC has the characteristics of high efficiency, quickness and simplicity and convenience in operation, and one sample can be analyzed in 9 minutes, so that the time is greatly saved, and the analysis efficiency is improved by nearly 3 times. The flow rate is increased to 0.6 mL / min, so that the analysis time of the sample is saved, and the detection of the sample is not interfered by impurities.

Description

technical field [0001] The invention relates to the technical field of determination of yew branches and leaves, in particular to a method for determining the content of 10-DABⅢ in yew branches and leaves. Background technique [0002] Yew is an ancient natural and rare anti-cancer plant, which belongs to the Taxus genus of Taxaceae (Taxaceae), and has a history of 2.5 million years on the earth. Southern yew is a unique species in my country, and it is the fastest growing and most widely distributed yew plant. Paclitaxel (Taxol), which is currently found in the southern yew, is one of the most effective anticancer substances recognized in the world. Paclitaxel is distributed in the roots, bark, stems and leaves of Taxus chinensis, but the content is limited. 10-Deacetylbaccatin III (10-DAB) is an index component of Taxus chinensis. It is rich in content and can be used to synthesize paclitaxel or more active docetaxel, which solves the problem of limited paclitaxel resour...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 蒋元烨曹勤韩吉林辉田继云李美莺冯明丽耿赟公培强
Owner SHANGHAI PUTUO DISTRICT CENT HOSPITAL